Thermozymes : Synthetic RNA thermometers based on ribozyme activity

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SARAGLIADIS, Athanasios, Stefanie S. KRAJEWSKI, Charlotte REHM, Franz NARBERHAUS, Jörg S. HARTIG, 2013. Thermozymes : Synthetic RNA thermometers based on ribozyme activity. In: RNA Biology. 10(6), pp. 1009-1016. ISSN 1547-6286. eISSN 1555-8584. Available under: doi: 10.4161/rna.24482

@article{Saragliadis2013-06Therm-26584, title={Thermozymes : Synthetic RNA thermometers based on ribozyme activity}, year={2013}, doi={10.4161/rna.24482}, number={6}, volume={10}, issn={1547-6286}, journal={RNA Biology}, pages={1009--1016}, author={Saragliadis, Athanasios and Krajewski, Stefanie S. and Rehm, Charlotte and Narberhaus, Franz and Hartig, Jörg S.} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:creator>Narberhaus, Franz</dc:creator> <dc:creator>Hartig, Jörg S.</dc:creator> <dc:contributor>Narberhaus, Franz</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Rehm, Charlotte</dc:creator> <dcterms:title>Thermozymes : Synthetic RNA thermometers based on ribozyme activity</dcterms:title> <dc:language>eng</dc:language> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:available rdf:datatype="">2014-02-26T13:35:30Z</dcterms:available> <dc:contributor>Krajewski, Stefanie S.</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>2013-06</dcterms:issued> <dcterms:abstract xml:lang="eng">Synthetic biology approaches often combine natural building blocks to generate new cellular activities. Here, we make use of two RNA elements to design a regulatory device with novel functionality. The system is based on a hammerhead ribozyme (HHR) that cleaves itself to generate a liberated ribosome-binding site and, thus, permits expression of a downstream gene. We connected a temperature-responsive RNA hairpin to the HHR and, thus, generated a temperature-controlled ribozyme that we call thermozyme. Specifically, a Salmonella RNA thermometer (RNAT) known to modulate small heat shock gene expression by temperature-controlled base-pairing and melting was fused to the ribozyme. Following an in vivo screening approach, we isolated two functional thermozymes. In vivo expression studies and in vitro structure probing experiments support a mechanism in which rising temperatures melt the thermometer structure impairing the self-cleavage reaction of the ribozyme. Since RNA cleavage is necessary to liberate the RBS, these engineered thermozymes shut off gene expression in response to a temperature increase and, thus, act in a reverse manner as the natural RNAT. Our results clearly emphasize the highly modular nature and biotechnological potential of ribozyme-based RNA thermometers.</dcterms:abstract> <dcterms:bibliographicCitation>RNA biology ; 10 (2013), 6. - S. 1009-1016</dcterms:bibliographicCitation> <dspace:hasBitstream rdf:resource=""/> <dc:contributor>Hartig, Jörg S.</dc:contributor> <dc:contributor>Rehm, Charlotte</dc:contributor> <dc:creator>Krajewski, Stefanie S.</dc:creator> <dcterms:rights rdf:resource=""/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Saragliadis, Athanasios</dc:contributor> <bibo:uri rdf:resource=""/> <dc:creator>Saragliadis, Athanasios</dc:creator> <dc:date rdf:datatype="">2014-02-26T13:35:30Z</dc:date> <dcterms:hasPart rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> </rdf:Description> </rdf:RDF>

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