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Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels

Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels

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Prüfsumme: MD5:2a437ea7a1082ca06673b4d9029d5646

BUHMANN, Matthias T., John G. DAY, Peter G. KROTH, 2013. Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels. In: Cryobiology. 67(1), pp. 23-29. ISSN 0011-2240. eISSN 1090-2392

@article{Buhmann2013-08Post--26338, title={Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels}, year={2013}, doi={10.1016/j.cryobiol.2013.04.005}, number={1}, volume={67}, issn={0011-2240}, journal={Cryobiology}, pages={23--29}, author={Buhmann, Matthias T. and Day, John G. and Kroth, Peter G.} }

2014-02-13T11:07:02Z Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels Day, John G. eng deposit-license Kroth, Peter G. 2014-02-13T11:07:02Z Buhmann, Matthias T. Cryobiology ; 67 (2013), 1. - S. 23-29 Buhmann, Matthias T. Over recent years, several planktonic and benthic freshwater diatom taxa have been established as laboratory model strains. In common with most freshwater diatoms the pennate diatom Planothidium frequentissimum suffers irreversible cell shrinkage on prolonged maintenance by serial transfers, without induction of the sexual cycle. Therefore, alternative strategies are required for the long-term maintenance of this strain. Conventional colligative cryopreservation approaches have previously proven unsuccessful with no regrowth. However, in this study using 5% dimethyl sulfoxide (Me<sub>2</sub>SO), controlled cooling at 1 °C min<sup>−1</sup>, automated ice seeding and cooling to −40 °C with a final plunge into liquid nitrogen, viability levels were enhanced from 0.3 ± 0.4% to 80 ± 3%, by incorporating a 48 h dark-recovery phase after rewarming. Omission, or reduction, of this recovery step resulted in obvious cell damage with photo-bleaching of pigments, indicative of oxidative-stress induced cell damage, with subsequent deterioration of cellular architecture. 2013-08 Day, John G. Kroth, Peter G.

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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