Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-257620 |
Author: | Ignatious Raja, Jennifer S.; Katanayeva, Natalya; Katanaev, Vladimir L.; Galizia, C. Giovanni |
Year of publication: | 2014 |
Published in: | European Journal of Neuroscience ; 39 (2014), 8. - pp. 1245-1255. - ISSN 0953-816X. - eISSN 1460-9568 |
Pubmed ID: | 24443946 |
DOI (citable link): | https://dx.doi.org/10.1111/ejn.12481 |
Summary: |
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we show that inhibiting Go in sensory neurons by pertussis toxin leads to behavioral deficits. We heterologously expressed the olfactory receptor dOr22a in human embryonic kidney cells (HEK293T). Stimulation with an odor led to calcium influx, which was amplified via calcium release from intracellular stores. Subsequent experiments indicated that the signaling was mediated by the Gβγ subunits of the heterotrimeric Go/i proteins. Finally, using in vivo calcium imaging, we show that Go and Gi contribute to odor responses both for the fast (phasic) as for the slow (tonic) response component. We propose a transduction cascade model involving several parallel processes, in which the metabotropic component is activated by Go and Gi, and uses Gβγ.
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Subject (DDC): | 570 Biosciences, Biology |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
IGNATIOUS RAJA, Jennifer S., Natalya KATANAYEVA, Vladimir L. KATANAEV, C. Giovanni GALIZIA, 2014. Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster. In: European Journal of Neuroscience. 39(8), pp. 1245-1255. ISSN 0953-816X. eISSN 1460-9568. Available under: doi: 10.1111/ejn.12481
@article{IgnatiousRaja2014-04subgr-25762, title={Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster}, year={2014}, doi={10.1111/ejn.12481}, number={8}, volume={39}, issn={0953-816X}, journal={European Journal of Neuroscience}, pages={1245--1255}, author={Ignatious Raja, Jennifer S. and Katanayeva, Natalya and Katanaev, Vladimir L. and Galizia, C. Giovanni} }
Galizia_257620.pdf | 126 |