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Role of G<sub>o/i</sub> subgroup of G proteins in olfactory signaling of Drosophila melanogaster

Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster

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IGNATIOUS RAJA, Jennifer S., Natalya KATANAYEVA, Vladimir L. KATANAEV, C. Giovanni GALIZIA, 2014. Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster. In: European Journal of Neuroscience. 39(8), pp. 1245-1255. ISSN 0953-816X. eISSN 1460-9568

@article{Ignatious Raja2014-04subgr-25762, title={Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster}, year={2014}, doi={10.1111/ejn.12481}, number={8}, volume={39}, issn={0953-816X}, journal={European Journal of Neuroscience}, pages={1245--1255}, author={Ignatious Raja, Jennifer S. and Katanayeva, Natalya and Katanaev, Vladimir L. and Galizia, C. Giovanni} }

2014-04 deposit-license European Journal of Neuroscience ; 39 (2014), 8. - S. 1245-1255 2014-01-24T09:18:47Z Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we show that inhibiting Go in sensory neurons by pertussis toxin leads to behavioral deficits. We heterologously expressed the olfactory receptor dOr22a in human embryonic kidney cells (HEK293T). Stimulation with an odor led to calcium influx, which was amplified via calcium release from intracellular stores. Subsequent experiments indicated that the signaling was mediated by the Gβγ subunits of the heterotrimeric Go/i proteins. Finally, using in vivo calcium imaging, we show that Go and Gi contribute to odor responses both for the fast (phasic) as for the slow (tonic) response component. We propose a transduction cascade model involving several parallel processes, in which the metabotropic component is activated by Go and Gi, and uses Gβγ. Ignatious Raja, Jennifer S. Katanaev, Vladimir L. 2014-01-24T09:18:47Z Galizia, C. Giovanni Katanaev, Vladimir L. Katanayeva, Natalya eng Katanayeva, Natalya Galizia, C. Giovanni Ignatious Raja, Jennifer S. Role of G<sub>o/i</sub> subgroup of G proteins in olfactory signaling of Drosophila melanogaster

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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