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Length-dependent conformational transitions of polyglutamine repeats as molecular origin of fibril initiation

Length-dependent conformational transitions of polyglutamine repeats as molecular origin of fibril initiation

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HECK, Benjamin, Franziska DOLL, Karin HAUSER, 2014. Length-dependent conformational transitions of polyglutamine repeats as molecular origin of fibril initiation. In: Biophysical Chemistry. 185, pp. 47-57. ISSN 0301-4622. eISSN 1873-4200

@article{Heck2014-01Lengt-25738, title={Length-dependent conformational transitions of polyglutamine repeats as molecular origin of fibril initiation}, year={2014}, doi={10.1016/j.bpc.2013.11.008}, volume={185}, issn={0301-4622}, journal={Biophysical Chemistry}, pages={47--57}, author={Heck, Benjamin and Doll, Franziska and Hauser, Karin} }

2014-02-07T11:46:52Z Hauser, Karin deposit-license Heck, Benjamin eng Heck, Benjamin Doll, Franziska Length-dependent conformational transitions of polyglutamine repeats as molecular origin of fibril initiation Polyglutamine (polyQ) sequences are found in a variety of proteins with normal function. However, their repeat expansion is associated with a number of neurodegenerative diseases, also called polyQ diseases. The length of the polyQ sequence, varying in the number of consecutive glutamines among different diseases, is critical for inducing fibril formation. We performed a systematic spectroscopic study to analyze the conformation of polyQ model peptides in dependence of the glutamine sequence lengths (K<sub>2</sub>Q<sub>n</sub>K<sub>2</sub> with n = 10, 20, 30). Complementary FTIR- and CD-spectra were measured in a wide concentration range and repeated heating and cooling cycles revealed the thermal stability of formed β-sheets. The shortest glutamine sequence K<sub>2</sub>Q<sub>10</sub>K<sub>2</sub> shows solely random structure for concentrations up to 10 mg/ml. By increasing the peptide length to K<sub>2</sub>Q<sub>20</sub>K<sub>2</sub>, a significant fraction of β-sheet is observed even at low concentrations of 0.01 mg/ml. The higher the concentration, the more the structural composition is dominated by the intermolecular β-sheet. The formation of highly thermostable β-sheet is much more pronounced in K<sub>2</sub>Q<sub>30</sub>K<sub>2</sub>. K<sub>2</sub>Q<sub>30</sub>K<sub>2</sub> precipitates at a concentration of 0.3 mg/ml. Our spectroscopic study shows that the aggregation tendency is enhanced with increased glutamine repeat expansion and that the concentration plays another critical factor in the β-sheet formation. 2014-02-07T11:46:52Z Hauser, Karin Biophysical Chemistry ; 185 (2014). - S. 47-57 2014-01 Doll, Franziska

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