DMD-based LED-Illumination Super-resolution and optical sectioning microscopy

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2013
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Dan, Dan
Yao, Baoli
Wang, Wen
Qi, Yujiao
Xia, Liang
Yan, Shaohui
Yang, Yanlong
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Scientific Reports. 2013, 3, 1116. eISSN 2045-2322. Available under: doi: 10.1038/srep01116
Zusammenfassung

Super-resolution three-dimensional (3D) optical microscopy has incomparable advantages over other high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. We present a novel approach of structured illumination microscopy (SIM) by using a digital micromirror device (DMD) for fringe projection and a low-coherence LED light for illumination. The lateral resolution of 90 nm and the optical sectioning depth of 120 μm were achieved. The maximum acquisition speed for 3D imaging in the optical sectioning mode was 1.6×107 pixels/second, which was mainly limited by the sensitivity and speed of the CCD camera. In contrast to other SIM techniques, the DMD-based LED-illumination SIM is cost-effective, ease of multi-wavelength switchable and speckle-noise-free. The 2D super-resolution and 3D optical sectioning modalities can be easily switched and applied to either fluorescent or non-fluorescent specimens.

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ISO 690DAN, Dan, Ming LEI, Baoli YAO, Wen WANG, Martin WINTERHALDER, Andreas ZUMBUSCH, Yujiao QI, Liang XIA, Shaohui YAN, Yanlong YANG, Peng GAO, Tong YE, Wei ZHAO, 2013. DMD-based LED-Illumination Super-resolution and optical sectioning microscopy. In: Scientific Reports. 2013, 3, 1116. eISSN 2045-2322. Available under: doi: 10.1038/srep01116
BibTex
@article{Dan2013DMDba-24786,
  year={2013},
  doi={10.1038/srep01116},
  title={DMD-based LED-Illumination Super-resolution and optical sectioning microscopy},
  volume={3},
  journal={Scientific Reports},
  author={Dan, Dan and Lei, Ming and Yao, Baoli and Wang, Wen and Winterhalder, Martin and Zumbusch, Andreas and Qi, Yujiao and Xia, Liang and Yan, Shaohui and Yang, Yanlong and Gao, Peng and Ye, Tong and Zhao, Wei},
  note={Article Number: 1116}
}
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