@article{Solis2013-09Reggi-24015,
  year={2013},
  doi={10.1091/mbc.E12-12-0854},
  title={Reggies/flotillins interact with Rab11a and SNX4 at the tubulo-vesicular recycling compartment and function in transferrin receptor and E-cadherin trafficking},
  number={17},
  volume={24},
  issn={1059-1524},
  journal={Molecular Biology of the Cell},
  pages={2689--2702},
  author={Solis, Gonzalo P. and Hülsbusch, Nikola and Radon, Yvonne and Katanaev, Vladimir L. and Plattner, Helmut and Stürmer, Claudia}
}

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    <dcterms:abstract xml:lang="eng">The lipid raft proteins reggie-1 and -2 (flotillins) were implicated in membrane protein trafficking but exactly how has remained elusive. We discovered that reggie-1 and -2 associate with the Rab11a, SNX4 and EHD1 decorated tubulo-vesicular recycling compartment in HeLa cells and that reggie-1 directly interacts with Rab11a and SNX4. shRNA-mediated down-regulation of reggie-1 (and -2) in HeLa cells reduced the association of Rab11a with tubular structures and impaired the recycling of the transferrin (Tf)-transferrin receptor (TfR) complex to the plasma membrane. Overexpression of constitutive-active Rab11a rescued TfR recycling in reggie-deficient HeLa cells. Likewise, in a Ca2+ switch assay in reggie-depleted A431 cells, internalized E-cadherin was not efficiently recycled to the plasma membrane upon Ca2+ repletion. However, E-cadherin recycling was rescued by overexpression of constitutive-active Rab11a or SNX4 in reggie-deficient A431 cells. This suggests that the function of reggie-1 in sorting and recycling occurs in association with Rab11a and SNX4. Interestingly, impaired recycling in reggie-deficient cells led to de novo E-cadherin biosynthesis and cell contact reformation, showing that cells have ways to compensate the loss of reggies. Together our results identify reggie-1 as a regulator of the Rab11a/SNX4-controlled sorting and recycling pathway which is -like reggies- evolutionarily conserved.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-16T12:26:02Z</dc:date>
    <dc:contributor>Solis, Gonzalo P.</dc:contributor>
    <dcterms:issued>2013-09</dcterms:issued>
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    <dc:creator>Hülsbusch, Nikola</dc:creator>
    <dcterms:title>Reggies/flotillins interact with Rab11a and SNX4 at the tubulo-vesicular recycling compartment and function in transferrin receptor and E-cadherin trafficking</dcterms:title>
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    <dc:contributor>Plattner, Helmut</dc:contributor>
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    <dc:contributor>Katanaev, Vladimir L.</dc:contributor>
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    <dc:creator>Radon, Yvonne</dc:creator>
    <dc:creator>Katanaev, Vladimir L.</dc:creator>
    <dc:creator>Solis, Gonzalo P.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-16T12:26:02Z</dcterms:available>
    <dcterms:bibliographicCitation>Molecular biology of the cell ; 24 (2013), 17. - S. 2689-2702</dcterms:bibliographicCitation>
    <dc:contributor>Hülsbusch, Nikola</dc:contributor>
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    <dc:contributor>Radon, Yvonne</dc:contributor>
    <dc:creator>Stürmer, Claudia</dc:creator>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <dc:creator>Plattner, Helmut</dc:creator>
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