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A hybrid genetic linkage map of two ecologically and morphologically divergent Midas cichlid fishes (Amphilophus spp.) obtained by massively parallel DNA sequencing (ddRADSeq)

A hybrid genetic linkage map of two ecologically and morphologically divergent Midas cichlid fishes (Amphilophus spp.) obtained by massively parallel DNA sequencing (ddRADSeq)

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RECKNAGEL, Hans, Kathryn R. ELMER, Axel MEYER, 2013. A hybrid genetic linkage map of two ecologically and morphologically divergent Midas cichlid fishes (Amphilophus spp.) obtained by massively parallel DNA sequencing (ddRADSeq). In: G3: Genes, Genomes, Genetics. 3(1), pp. 65-74. eISSN 2160-1836

@article{Recknagel2013-01hybri-23676, title={A hybrid genetic linkage map of two ecologically and morphologically divergent Midas cichlid fishes (Amphilophus spp.) obtained by massively parallel DNA sequencing (ddRADSeq)}, year={2013}, doi={10.1534/g3.112.003897}, number={1}, volume={3}, journal={G3: Genes, Genomes, Genetics}, pages={65--74}, author={Recknagel, Hans and Elmer, Kathryn R. and Meyer, Axel} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/23676"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-06-17T12:54:33Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/23676"/> <dcterms:title>A hybrid genetic linkage map of two ecologically and morphologically divergent Midas cichlid fishes (Amphilophus spp.) obtained by massively parallel DNA sequencing (ddRADSeq)</dcterms:title> <dc:creator>Meyer, Axel</dc:creator> <dcterms:bibliographicCitation>G3: Genes, genomes, genetics ; 3 (2013), 1. - S. 65-74</dcterms:bibliographicCitation> <dcterms:issued>2013-01</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:creator>Elmer, Kathryn R.</dc:creator> <dc:creator>Recknagel, Hans</dc:creator> <dc:contributor>Meyer, Axel</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-06-17T12:54:33Z</dcterms:available> <dc:contributor>Recknagel, Hans</dc:contributor> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">Cichlid fishes are an excellent model system for studying speciation and the formation of adaptive radiations because of their tremendous species richness and astonishing phenotypic diversity. Most research has focused on African rift lake fishes, although Neotropical cichlid species display much variability as well. Almost one dozen species of the Midas cichlid species complex (Amphilophus spp.) have been described so far and have formed repeated adaptive radiations in several Nicaraguan crater lakes. Here we apply double-digest restriction-site associated DNA sequencing to obtain a high-density linkage map of an interspecific cross between the benthic Amphilophus astorquii and the limnetic Amphilophus zaliosus, which are sympatric species endemic to Crater Lake Apoyo, Nicaragua. A total of 755 RAD markers were genotyped in 343 F(2) hybrids. The map resolved 25 linkage groups and spans a total distance of 1427 cM with an average marker spacing distance of 1.95 cM, almost matching the total number of chromosomes (n = 24) in these species. Regions of segregation distortion were identified in five linkage groups. Based on the pedigree of parents to F(2) offspring, we calculated a genome-wide mutation rate of 6.6 × 10(-8) mutations per nucleotide per generation. This genetic map will facilitate the mapping of ecomorphologically relevant adaptive traits in the repeated phenotypes that evolved within the Midas cichlid lineage and, as the first linkage map of a Neotropical cichlid, facilitate comparative genomic analyses between African cichlids, Neotropical cichlids and other teleost fishes.</dcterms:abstract> <dc:contributor>Elmer, Kathryn R.</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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