Image Analysis of Nuclear Envelope Breakdown Events using KNIME

Publikationstyp: Beitrag zu einer Konferenz
URI (zitierfähiger Link): http://nbn-resolving.de/urn:nbn:de:bsz:352-224337
Autor/innen: Rieß, Thorsten; Marino, Joseph; Wandke, Cornelia; Merhof, Dorit; Deussen, Oliver; Csucs, Gabor; Kutay, Ulrike; Horvath, Peter
Erscheinungsjahr: 2012
Konferenz: WCSB, 4. Jun 2012 - 6. Jun 2012, Ulm, Germany
Erschienen in: Ninth International Workshop on Computational Systems Biology, WCSB 2012, June 4 - 6, 2012, Ulm, Germany / Narjo, Antti et al. (Hrsg.). - Tampere : Tampere International Center for Signal Processing, 2012. - (TICSP series ; 61). - S. 67-70. - ISBN 978-952-15-2853-8
Zusammenfassung:
Insights into the molecular mechanism underlying nuclear envelope breakdown (NEBD) are crucial to understand dynamic changes of the nuclear envelope (NE) that occur at mitotic entry of eukaryotic cells. In this paper, we present an image processing algorithm and its implementation in KNIME, which allows to automatically quantify image data obtained from the in vitro NEBD assay. The algorithm consists of image alignment via phase correlation, nucleus identification using adaptive thresholding, morphological operations, and intensity measurements. The results of these measurements are verified using images from a known nuclear envelope disassembly assay.
Fachgebiet (DDC): 004 Informatik
Link zur Lizenz: Deposit-Lizenz
Universitätsbibliographie: Ja

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RIESS, Thorsten, Joseph MARINO, Cornelia WANDKE, Dorit MERHOF, Oliver DEUSSEN, Gabor CSUCS, Ulrike KUTAY, Peter HORVATH, 2012. Image Analysis of Nuclear Envelope Breakdown Events using KNIME. WCSB. Ulm, Germany, 4. Jun 2012 - 6. Jun 2012. In: NARJO, Antti, ed. and others. Ninth International Workshop on Computational Systems Biology, WCSB 2012, June 4 - 6, 2012, Ulm, Germany. WCSB. Ulm, Germany, 4. Jun 2012 - 6. Jun 2012. Tampere:Tampere International Center for Signal Processing, pp. 67-70. ISBN 978-952-15-2853-8

@inproceedings{Rie2012Image-22433, title={Image Analysis of Nuclear Envelope Breakdown Events using KNIME}, year={2012}, number={61}, isbn={978-952-15-2853-8}, address={Tampere}, publisher={Tampere International Center for Signal Processing}, series={TICSP series}, booktitle={Ninth International Workshop on Computational Systems Biology, WCSB 2012, June 4 - 6, 2012, Ulm, Germany}, pages={67--70}, editor={Narjo, Antti}, author={Rieß, Thorsten and Marino, Joseph and Wandke, Cornelia and Merhof, Dorit and Deussen, Oliver and Csucs, Gabor and Kutay, Ulrike and Horvath, Peter} }

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