Automated Image Processing to Quantify Cell Migration
| Type of Publication: | Contribution to a conference |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-224037 |
| Author: | Shen, Minmin; Zimmer, Bastian; Leist, Marcel; Merhof, Dorit |
| Year of publication: | 2013 |
| Published in: | Bildverarbeitung für die Medizin 2013 / Meinzer, Hans-Peter; Deserno, Thomas Martin; Handels, Heinz; Tolxdorff, Thomas (ed.). - Berlin, Heidelberg : Springer Berlin Heidelberg, 2013. - (Informatik aktuell). - pp. 152-157. - ISBN 978-3-642-36479-2 |
| DOI (citable link): | https://dx.doi.org/10.1007/978-3-642-36480-8_28 |
| Summary: |
Methods to evaluate migration capacity of stem cells and the inhibition by chemicals are important for biomedical research. Here, we established an automated image processing framework to quantify migration of human neural crest (NC) cells into an initially empty, circular region of interest (ROI). The ROI is partially filled during the experiment by migrating cells. Based on an image captured only once at the end of the biological experiment, the framework identifies the initial ROI. The identification worked also, when the distribution of surrounding cells showed large heterogeneity. After segmentation, the number of migrated cells was identified. The image processing framework was capable of efficiently quantifying chemical effects on cell migration.
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| Subject (DDC): | 004 Computer Science |
| Link to License: | Terms of use |
| Bibliography of Konstanz: | Yes |
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SHEN, Minmin, Bastian ZIMMER, Marcel LEIST, Dorit MERHOF, 2013. Automated Image Processing to Quantify Cell Migration. In: MEINZER, Hans-Peter, ed., Thomas Martin DESERNO, ed., Heinz HANDELS, ed., Thomas TOLXDORFF, ed.. Bildverarbeitung für die Medizin 2013. Berlin, Heidelberg:Springer Berlin Heidelberg, pp. 152-157. ISBN 978-3-642-36479-2. Available under: doi: 10.1007/978-3-642-36480-8_28
@inproceedings{Shen2013-02-20Autom-22403, title={Automated Image Processing to Quantify Cell Migration}, year={2013}, doi={10.1007/978-3-642-36480-8_28}, isbn={978-3-642-36479-2}, address={Berlin, Heidelberg}, publisher={Springer Berlin Heidelberg}, series={Informatik aktuell}, booktitle={Bildverarbeitung für die Medizin 2013}, pages={152--157}, editor={Meinzer, Hans-Peter and Deserno, Thomas Martin and Handels, Heinz and Tolxdorff, Thomas}, author={Shen, Minmin and Zimmer, Bastian and Leist, Marcel and Merhof, Dorit} }
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