KOPS - Das Institutionelle Repositorium der Universität Konstanz

Preferential positive selection of V<sub>α</sub>2<sup>+</sup>CD8<sup>+</sup> T cells in mouse strains expressing both H-2<sup>k</sup>kand T cell receptor V<sub>α</sub><sup>a</sup> haplotypes : determination with a V<sub>α</sub>2-specific monoclonal antibody

Preferential positive selection of Vα2+CD8+ T cells in mouse strains expressing both H-2kkand T cell receptor Vαa haplotypes : determination with a Vα2-specific monoclonal antibody

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

PIRCHER, Hanspeter, Najet REBAI, Marcus GRÖTTRUP, Claude GRÉGOIRE, Daniel E. SPEISER, Mary Patt HAPP, Ed PALMER, Rolf M. ZINKERNAGEL, Hans HENGARTNER, Bernard MALISSEN, 1992. Preferential positive selection of Vα2+CD8+ T cells in mouse strains expressing both H-2kkand T cell receptor Vαa haplotypes : determination with a Vα2-specific monoclonal antibody. In: European Journal of Immunology. 22(2), pp. 399-404. ISSN 0014-2980. eISSN 1521-4141

@article{Pircher1992-02Prefe-22366, title={Preferential positive selection of Vα2+CD8+ T cells in mouse strains expressing both H-2kkand T cell receptor Vαa haplotypes : determination with a Vα2-specific monoclonal antibody}, year={1992}, doi={10.1002/eji.1830220217}, number={2}, volume={22}, issn={0014-2980}, journal={European Journal of Immunology}, pages={399--404}, author={Pircher, Hanspeter and Rebai, Najet and Gröttrup, Marcus and Grégoire, Claude and Speiser, Daniel E. and Happ, Mary Patt and Palmer, Ed and Zinkernagel, Rolf M. and Hengartner, Hans and Malissen, Bernard} }

Hengartner, Hans Malissen, Bernard Pircher, Hanspeter eng Hengartner, Hans Speiser, Daniel E. A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specific for murine TcR V alpha 2 chains. The V alpha 2+ T cell population was examined in various inbred strains by two-color immunofluorescence using B20.1 and CD4- and CD8-specific antibodies with the following results: (a) the B20.1 antibody detected most members of the TcR V alpha 2 subfamily in the four TcR V alpha haplotypes tested; (b) in most strains examined, TcR V alpha 2 expression was biased to the CD4 subset (7.4%-17.4% V alpha 2+ T cells) as compared to the CD8 compartment (3.8%-13.3%); (c) TcR V alpha 2 expression was not influenced by Mls gene products and (d) increased positive selection of V alpha 2+ CD8+ T cells by H-2k major histocompatibility complex molecules occurred in all murine strains tested of the TcR V alpha a, but not in those bearing the TcR V alpha b haplotype. Rebai, Najet 1992-02 Pircher, Hanspeter 2013-04-03T09:03:01Z Malissen, Bernard Grégoire, Claude 2013-04-03T09:03:01Z Palmer, Ed Palmer, Ed Happ, Mary Patt deposit-license Grégoire, Claude Gröttrup, Marcus Speiser, Daniel E. Rebai, Najet European Journal of Immunology ; 22 (1992), 2. - S. 399-404 Preferential positive selection of V<sub>α</sub>2<sup>+</sup>CD8<sup>+</sup> T cells in mouse strains expressing both H-2<sup>k</sup>kand T cell receptor V<sub>α</sub><sup>a</sup> haplotypes : determination with a V<sub>α</sub>2-specific monoclonal antibody Happ, Mary Patt Zinkernagel, Rolf M. Gröttrup, Marcus Zinkernagel, Rolf M.

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto