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Molecular analysis of T-cell receptor transcripts in a human T-cell leukemia bearing a t(1;14) and an inv(7); cell surface expression of a TCR-ß chain in the absence of alpha chain

Molecular analysis of T-cell receptor transcripts in a human T-cell leukemia bearing a t(1;14) and an inv(7); cell surface expression of a TCR-ß chain in the absence of alpha chain

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BERNARD, Olivier, Marcus GRÖTTRUP, Francine MUGNERET, Roland BERGER, Orly AZOGUI, 1993. Molecular analysis of T-cell receptor transcripts in a human T-cell leukemia bearing a t(1;14) and an inv(7); cell surface expression of a TCR-ß chain in the absence of alpha chain. In: Leukemia. 7(10), pp. 1645-1653. ISSN 0887-6924. eISSN 1476-5551

@article{Bernard1993Molec-22359, title={Molecular analysis of T-cell receptor transcripts in a human T-cell leukemia bearing a t(1;14) and an inv(7); cell surface expression of a TCR-ß chain in the absence of alpha chain}, year={1993}, number={10}, volume={7}, issn={0887-6924}, journal={Leukemia}, pages={1645--1653}, author={Bernard, Olivier and Gröttrup, Marcus and Mugneret, Francine and Berger, Roland and Azogui, Orly} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22359"> <dc:language>eng</dc:language> <dc:contributor>Bernard, Olivier</dc:contributor> <dc:creator>Mugneret, Francine</dc:creator> <dc:creator>Azogui, Orly</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-04-16T10:12:35Z</dcterms:available> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-04-16T10:12:35Z</dc:date> <dcterms:title>Molecular analysis of T-cell receptor transcripts in a human T-cell leukemia bearing a t(1;14) and an inv(7); cell surface expression of a TCR-ß chain in the absence of alpha chain</dcterms:title> <dcterms:issued>1993</dcterms:issued> <dc:contributor>Berger, Roland</dc:contributor> <dc:creator>Bernard, Olivier</dc:creator> <dcterms:abstract xml:lang="eng">The polymerase chain reaction (PCR) was used to amplify cDNA in order to characterize normal and hybrid T-cell receptor (TCR) gene rearrangements derived from a T-cell acute lymphoblastic leukemia (T-ALL) bearing a chromosome 7 inversion. The nucleotide sequence analysis of the amplified product showed the presence of an out-of-frame V beta/J gamma/C gamma transcript and an in-frame V gamma/J gamma/C beta transcript which result from an interlocus recombination between the TCR-beta and gamma loci and the transcription of the reciprocal hybrid TCR gene. The sequence analysis of the reciprocal DNA segment directly involved in the breakpoint of the inversion showed a recombination between a J gamma-sequence heptamer signal and a coding J beta gene segment. The exonuclease nibbling of each DNA extremity and the non-templated nucleotide insertion observed at both coding and reciprocal joints demonstrate that the inversion is mediated by the lymphocyte recombinase complex. During T-cell differentiation, TCR-beta genes are rearranged prior to TCR-alpha genes. In the present case of T-ALL, we have shown that TCR-delta genes are rearranged at both loci, excluding productive rearrangements of TCR-alpha genes. The molecular analysis of the normal TCR genes derived from the leukemic cells revealed the presence of productively rearranged TCR-beta, gamma, and delta genes. Cell surface staining of these cells showed the presence of CD3 molecules and of a TCR-beta chain corresponding to the normal beta allele expressed in a disulfide-linked complex with a protein which is not TCR-alpha, gamma, or delta. This could represent the cell surface expression of the hybrid TCR-V gamma/C beta protein or the expression of a TCR-beta homodimer.</dcterms:abstract> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dc:contributor>Azogui, Orly</dc:contributor> <dc:creator>Berger, Roland</dc:creator> <dc:contributor>Mugneret, Francine</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22359"/> <dcterms:bibliographicCitation>Leukemia ; 7 (1993), 10. - S. 1645-1653</dcterms:bibliographicCitation> <dc:creator>Gröttrup, Marcus</dc:creator> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

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