Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters
| Type of Publication: | Journal article |
| Author: | Kesberg, Anna Isabella; Schleheck, David |
| Year of publication: | 2013 |
| Published in: | Journal of Microbiological Methods ; 93 (2013), 1. - pp. 55-57. - ISSN 0167-7012. - eISSN 1872-8359 |
| Pubmed ID: | 23419826 |
| DOI (citable link): | https://dx.doi.org/10.1016/j.mimet.2013.02.001 |
| Summary: |
Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Bibliography of Konstanz: | Yes |
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KESBERG, Anna Isabella, David SCHLEHECK, 2013. Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters. In: Journal of Microbiological Methods. 93(1), pp. 55-57. ISSN 0167-7012. eISSN 1872-8359. Available under: doi: 10.1016/j.mimet.2013.02.001
@article{Kesberg2013-04Impro-22327, title={Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters}, year={2013}, doi={10.1016/j.mimet.2013.02.001}, number={1}, volume={93}, issn={0167-7012}, journal={Journal of Microbiological Methods}, pages={55--57}, author={Kesberg, Anna Isabella and Schleheck, David} }
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