KOPS - The Institutional Repository of the University of Konstanz

Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters

Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

KESBERG, Anna Isabella, David SCHLEHECK, 2013. Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters. In: Journal of Microbiological Methods. 93(1), pp. 55-57. ISSN 0167-7012. eISSN 1872-8359. Available under: doi: 10.1016/j.mimet.2013.02.001

@article{Kesberg2013-04Impro-22327, title={Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters}, year={2013}, doi={10.1016/j.mimet.2013.02.001}, number={1}, volume={93}, issn={0167-7012}, journal={Journal of Microbiological Methods}, pages={55--57}, author={Kesberg, Anna Isabella and Schleheck, David} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22327"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-11T10:03:08Z</dcterms:available> <dc:contributor>Kesberg, Anna Isabella</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Schleheck, David</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:bibliographicCitation>Journal of Microbiological Methods ; 93 (2013), 1. - S. 55-57</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22327"/> <dcterms:issued>2013-04</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Kesberg, Anna Isabella</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Schleheck, David</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-11T10:03:08Z</dc:date> <dc:rights>terms-of-use</dc:rights> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>Improved protocol for recovery of bacterial DNA from water filters : Sonication and backflushing of commercial syringe filters</dcterms:title> <dcterms:abstract xml:lang="eng">Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial "syringe filters", involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h.</dcterms:abstract> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


Browse

My Account