Type of Publication: | Journal article |
Author: | Macagno, Annalisa; Gilliet, Michel; Sallusto, Federica; Lanzavecchia, Antonio; Nestle, Frank O.; Gröttrup, Marcus |
Year of publication: | 1999 |
Published in: | European Journal of Immunology ; 29 (1999), 12. - pp. 4037-4042. - ISSN 0014-2980. - eISSN 1521-4141 |
DOI (citable link): | https://dx.doi.org/10.1002/(SICI)1521-4141(199912)29:12<4037::AID-IMMU4037>3.0.CO;2-T |
Summary: |
Dendritic cells (DC) are highly specialized professional antigen presenting cells which are pivotal for the initiation and control of the cytotoxic T cell response. Upon stimulation by cytokines, bacteria, or CD40L DC undergo a maturation process from an antigen-receptive state to a state of optimal stimulation of T cells. We investigated the composition of proteasomes of DC derived from human peripheral blood monocytes before and after stimulation by CD40L, LPS, or proinflammatory cytokines (TNF-alpha + IL-6 + IL-1beta). Immunoprecipitation of proteasomes and analysis on two-dimensional gels revealed that during maturation the inducible proteasome subunits LMP2, LMP7, and MECL-1 are up-regulated and that the neosynthesis of proteasomes is switched exclusively to the production of immunoproteasomes containing these subunits. The proteasome regulator PA28 is markedly up-regulated in mature DC and in addition a so - far unidentified 21-kDa protein co-precipitates with the proteasome in LPS - stimulated DC. These changes in proteasome composition may be functionally linked to special properties of DC like MHC class I up-regulation or cross-priming. Our findings imply that the spectrum of class I-bound peptides may change after DC maturation which could be relevant for the design of DC - based vaccines.
|
Subject (DDC): | 570 Biosciences, Biology |
Keywords: | Antigen presentation, TNF- alpha, CD40, Lipopolysaccharide, LMP2 |
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MACAGNO, Annalisa, Michel GILLIET, Federica SALLUSTO, Antonio LANZAVECCHIA, Frank O. NESTLE, Marcus GRÖTTRUP, 1999. Dendritic cells up-regulate immunoproteasomes and the proteasome regulator PA28 during maturation. In: European Journal of Immunology. 29(12), pp. 4037-4042. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/(SICI)1521-4141(199912)29:12<4037::AID-IMMU4037>3.0.CO;2-T
@article{Macagno1999Dendr-22233, title={Dendritic cells up-regulate immunoproteasomes and the proteasome regulator PA28 during maturation}, year={1999}, doi={10.1002/(SICI)1521-4141(199912)29:12<4037::AID-IMMU4037>3.0.CO;2-T}, number={12}, volume={29}, issn={0014-2980}, journal={European Journal of Immunology}, pages={4037--4042}, author={Macagno, Annalisa and Gilliet, Michel and Sallusto, Federica and Lanzavecchia, Antonio and Nestle, Frank O. and Gröttrup, Marcus} }
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