KOPS - Das Institutionelle Repositorium der Universität Konstanz

Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20 S proteasome

Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20 S proteasome

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

SCHMIDTKE, Gunter, Sabine EMCH, Marcus GRÖTTRUP, Hermann-Georg HOLZHÜTTER, 2000. Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20 S proteasome. In: Journal of Biological Chemistry. 275(29), pp. 22056-22063. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M002513200

@article{Schmidtke2000-07-21Evide-22167, title={Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20 S proteasome}, year={2000}, doi={10.1074/jbc.M002513200}, number={29}, volume={275}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={22056--22063}, author={Schmidtke, Gunter and Emch, Sabine and Gröttrup, Marcus and Holzhütter, Hermann-Georg} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22167"> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Emch, Sabine</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22167"/> <dc:contributor>Emch, Sabine</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-08T11:00:06Z</dcterms:available> <dc:language>eng</dc:language> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dc:creator>Schmidtke, Gunter</dc:creator> <dc:contributor>Schmidtke, Gunter</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>Evidence for the existence of a non-catalytic modifier site of peptide hydrolysis by the 20 S proteasome</dcterms:title> <dc:contributor>Holzhütter, Hermann-Georg</dc:contributor> <dcterms:abstract xml:lang="eng">The 20 S proteasome is an endoprotease complex that preferentially cleaves peptides C-terminal of hydrophobic, basic, and acidic residues. Recently, we showed that these specific activities, classified as chymotrypsin-like, trypsin-like, and peptidylglutamyl peptide-hydrolyzing (PGPH) activity, are differently affected by Ritonavir, an inhibitor of human immunodeficiency virus-1 protease. Ritonavir competitively inhibited the chymotrypsin-like activity, whereas the trypsin-like activity was enhanced. Here we demonstrate that the Ritonavir-mediated up-regulation of the trypsin-like activity is not affected by specific active site inhibitors of the chymo-trypsin-like and PGPH activity. Moreover, we show that the mutual regulation of chymotrypsin-like and PGPH activities by their substrates as described previously by a "cyclical bite-chew" model is not affected by selective inhibitors of the respective active sites. These data challenge the bite-chew model and suggest that effectors of proteasome activity can act by binding to non-catalytic sites. Accordingly, we propose a kinetic "two-site modifier" model that assumes that the substrate (or effector) may bind to an active site as well as to a second non-catalytic modifier site. This model appears to be valid as it describes the complex kinetic effects of Ritonavir very well. Since Ritonavir partially inhibits major histocompatibility complex class I restricted antigen presentation, the postulated modifier site may be required to coordinate the active centers of the proteasome for the production of class I peptide ligands.</dcterms:abstract> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-08T11:00:06Z</dc:date> <dc:rights>deposit-license</dc:rights> <dcterms:issued>2000-07-21</dcterms:issued> <dc:creator>Holzhütter, Hermann-Georg</dc:creator> <dcterms:bibliographicCitation>The journal of biological chemistry : JBC ; 275 (2000), 29. - S. 22056-22063</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Gröttrup, Marcus</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto