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Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope

Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope

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SCHWARZ, Katrin, Maries VAN DEN BROEK, Susanne KOSTKA, Regine KRAFT, Andrea SOZA, Gunter SCHMIDTKE, Peter KLÖTZEL, Marcus GRÖTTRUP, 2000. Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope. In: Journal of Immunology. 165(2), pp. 768-778. ISSN 0022-1767. eISSN 1550-6606

@article{Schwarz2000Overe-22164, title={Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope}, year={2000}, doi={10.4049/jimmunol.165.2.768}, number={2}, volume={165}, issn={0022-1767}, journal={Journal of Immunology}, pages={768--778}, author={Schwarz, Katrin and Van den Broek, Maries and Kostka, Susanne and Kraft, Regine and Soza, Andrea and Schmidtke, Gunter and Klötzel, Peter and Gröttrup, Marcus} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22164"> <dcterms:issued>2000</dcterms:issued> <dc:contributor>Schwarz, Katrin</dc:contributor> <dc:contributor>Kraft, Regine</dc:contributor> <dc:contributor>Klötzel, Peter</dc:contributor> <dc:contributor>Kostka, Susanne</dc:contributor> <dc:contributor>Soza, Andrea</dc:contributor> <dc:creator>Gröttrup, Marcus</dc:creator> <dcterms:abstract xml:lang="eng">The proteasome is a large protease complex that generates most of the peptide ligands of MHC class I molecules either in their final form or in the form of N-terminally extended precursors. Upon the stimulation of cells with IFN-gamma, three constitutively expressed subunits of the 20S proteasome are replaced by the inducible subunits LMP2 (low-molecular mass polypeptide 2), LMP7, and MECL-1 (multicatalytic endopeptidase complex-like-1) to form so-called immunoproteasomes. We show in this study that overexpression of these three subunits in triple transfectants led to a marked enhancement in the H-2Ld-restricted presentation of the immunodominant nonameric epitope NP118, which is derived from the nucleoprotein (NP) of lymphocytic choriomeningitis virus. Overexpression of the alpha and beta subunits of the IFN-gamma-inducible proteasome regulator PA28, in contrast, did not have a comparable effect. In vitro, immunoproteasomes as compared with constitutive proteasomes generated higher amounts of 11- and 12-mer fragments containing the NP118 epitope. These are likely to be cytosolic precursors of NP118, as a proline anchor residue in the second position of NP118 may interfere with TAP-mediated transport of the nonameric epitope itself. In conclusion, we provide evidence that up-regulation of the three inducible subunits, LMP2, LMP7, and MECL-1, can result in a marked improvement of Ag presentation and that, depending on the epitope, PA28 and immunoproteasomes may differentially affect Ag processing.</dcterms:abstract> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dc:creator>Klötzel, Peter</dc:creator> <dc:creator>Van den Broek, Maries</dc:creator> <dc:creator>Schmidtke, Gunter</dc:creator> <dcterms:title>Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope</dcterms:title> <dcterms:bibliographicCitation>Journal of Immunology ; 165 (2000), 2. - S. 768-778</dcterms:bibliographicCitation> <dc:creator>Schwarz, Katrin</dc:creator> <dc:contributor>Schmidtke, Gunter</dc:contributor> <dc:contributor>Van den Broek, Maries</dc:contributor> <dc:creator>Soza, Andrea</dc:creator> <dc:creator>Kostka, Susanne</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-27T09:43:40Z</dc:date> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Kraft, Regine</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22164"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-27T09:43:40Z</dcterms:available> </rdf:Description> </rdf:RDF>

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