KOPS - Das Institutionelle Repositorium der Universität Konstanz

The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition

The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:528abf8ea515dfc3c019090cce1e02e2

SCHWARZ, Katrin, Maren EGGERS, Andrea SOZA, Ulrich H. KOSZINOWSKI, Peter-M. KLÖTZEL, Marcus GRÖTTRUP, 2000. The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition. In: European Journal of Immunology. 30(12), pp. 3672-3679. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-B

@article{Schwarz2000prote-22161, title={The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition}, year={2000}, doi={10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-B}, number={12}, volume={30}, issn={0014-2980}, journal={European Journal of Immunology}, pages={3672--3679}, author={Schwarz, Katrin and Eggers, Maren and Soza, Andrea and Koszinowski, Ulrich H. and Klötzel, Peter-M. and Gröttrup, Marcus} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22161"> <dc:contributor>Schwarz, Katrin</dc:contributor> <dc:contributor>Soza, Andrea</dc:contributor> <dc:creator>Eggers, Maren</dc:creator> <dc:rights>terms-of-use</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22161"/> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dc:contributor>Koszinowski, Ulrich H.</dc:contributor> <dc:creator>Schwarz, Katrin</dc:creator> <dc:creator>Soza, Andrea</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:bibliographicCitation>European journal of immunology ; 30 (2000), 12. - S. 3672-3679</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-15T13:34:10Z</dcterms:available> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Gröttrup, Marcus</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-15T13:34:10Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/22161/2/Schwarz_221615.pdf"/> <dcterms:issued>2000</dcterms:issued> <dcterms:abstract xml:lang="eng">PA28α/β is a regulatory complex of the 20S proteasome which consists of two IFN-γ inducible subunits. Both subunits, α and β, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28α subunit enhanced the MHC class I-restricted presentation of two viral epitopes and that purified PA28α/β accelerated T cell epitope generation by the 20S proteasome in vitro, indicating a role for PA28α/β in antigen presentation. This conclusion was recently confirmed in PA28β gene targeted mice which were severely deficient in MHC class I-restricted antigen presentation. These mice displayed a defect in the assembly of immunoproteasomes, suggesting that a lack of the proteasome subunits LMP2, LMP7, and MECL-1 may account for the deficiency in antigen presentation. In this study we investigated whether the effect of PA28α/β on antigen presentation is dependent on a change of proteasome subunit composition. We have analyzed the assembly and subunit composition of proteasomes in fibroblast transfectants overexpressing both, α and β subunits of PA28. In these transfectants we found a marked enhancement in the presentation of the immunodominant H-2Ld-restricted pp89 epitope of murine cytomegalovirus, although the 20S proteasome composition was the same as in recipient cells. We, therefore, conclude that PA28α/β can enhance antigen processing independently of changes in 20S proteasome subunit composition or assembly.</dcterms:abstract> <dcterms:title>The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Eggers, Maren</dc:contributor> <dc:contributor>Klötzel, Peter-M.</dc:contributor> <dc:language>eng</dc:language> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Klötzel, Peter-M.</dc:creator> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/22161/2/Schwarz_221615.pdf"/> <dc:creator>Koszinowski, Ulrich H.</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Schwarz_221615.pdf 20

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto