@article{Schwarz2000prote-22161,
  year={2000},
  doi={10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-B},
  title={The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition},
  number={12},
  volume={30},
  issn={0014-2980},
  journal={European Journal of Immunology},
  pages={3672--3679},
  author={Schwarz, Katrin and Eggers, Maren and Soza, Andrea and Koszinowski, Ulrich H. and Klötzel, Peter-M. and Gröttrup, Marcus}
}

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    <dc:creator>Soza, Andrea</dc:creator>
    <dc:creator>Schwarz, Katrin</dc:creator>
    <dcterms:title>The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition</dcterms:title>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-03-15T13:34:10Z</dc:date>
    <dc:contributor>Klötzel, Peter-M.</dc:contributor>
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    <dc:creator>Eggers, Maren</dc:creator>
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    <dcterms:issued>2000</dcterms:issued>
    <dc:language>eng</dc:language>
    <dc:contributor>Soza, Andrea</dc:contributor>
    <dc:creator>Koszinowski, Ulrich H.</dc:creator>
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    <dc:contributor>Koszinowski, Ulrich H.</dc:contributor>
    <dcterms:abstract xml:lang="eng">PA28α/β is a regulatory complex of the 20S proteasome which consists of two IFN-γ inducible subunits. Both subunits, α and β, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28α subunit enhanced the MHC class I-restricted presentation of two viral epitopes and that purified PA28α/β accelerated T cell epitope generation by the 20S proteasome in vitro, indicating a role for PA28α/β in antigen presentation. This conclusion was recently confirmed in PA28β gene targeted mice which were severely deficient in MHC class I-restricted antigen presentation. These mice displayed a defect in the assembly of immunoproteasomes, suggesting that a lack of the proteasome subunits LMP2, LMP7, and MECL-1 may account for the deficiency in antigen presentation. In this study we investigated whether the effect of PA28α/β on antigen presentation is dependent on a change of proteasome subunit composition. We have analyzed the assembly and subunit composition of proteasomes in fibroblast transfectants overexpressing both, α and β subunits of PA28. In these transfectants we found a marked enhancement in the presentation of the immunodominant H-2Ld-restricted pp89 epitope of murine cytomegalovirus, although the 20S proteasome composition was the same as in recipient cells. We, therefore, conclude that PA28α/β can enhance antigen processing independently of changes in 20S proteasome subunit composition or assembly.</dcterms:abstract>
    <dc:creator>Gröttrup, Marcus</dc:creator>
    <dc:contributor>Gröttrup, Marcus</dc:contributor>
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    <dcterms:bibliographicCitation>European journal of immunology ; 30 (2000), 12. - S. 3672-3679</dcterms:bibliographicCitation>
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    <dc:contributor>Schwarz, Katrin</dc:contributor>
    <dc:contributor>Eggers, Maren</dc:contributor>
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    <dc:creator>Klötzel, Peter-M.</dc:creator>
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