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The UBA Domains of NUB1L Are Required for Binding but Not for Accelerated Degradation of the Ubiquitin-like Modifier FAT10

The UBA Domains of NUB1L Are Required for Binding but Not for Accelerated Degradation of the Ubiquitin-like Modifier FAT10

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SCHMIDTKE, Gunter, Birte KALVERAM, Elvira WEBER, Petra BOCHTLER, Sebastian LUKASIAK, Mark-Steffen HIPP, Marcus GRÖTTRUP, 2006. The UBA Domains of NUB1L Are Required for Binding but Not for Accelerated Degradation of the Ubiquitin-like Modifier FAT10. In: The Journal of Biological Chemistry. 281(29), pp. 20045-20054. ISSN 0021-9258. eISSN 1083-351X

@article{Schmidtke2006Domai-22113, title={The UBA Domains of NUB1L Are Required for Binding but Not for Accelerated Degradation of the Ubiquitin-like Modifier FAT10}, year={2006}, doi={10.1074/jbc.M603063200}, number={29}, volume={281}, issn={0021-9258}, journal={The Journal of Biological Chemistry}, pages={20045--20054}, author={Schmidtke, Gunter and Kalveram, Birte and Weber, Elvira and Bochtler, Petra and Lukasiak, Sebastian and Hipp, Mark-Steffen and Gröttrup, Marcus} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/22113"> <dcterms:issued>2006</dcterms:issued> <dc:contributor>Hipp, Mark-Steffen</dc:contributor> <dc:creator>Gröttrup, Marcus</dc:creator> <dc:creator>Weber, Elvira</dc:creator> <dcterms:title>The UBA Domains of NUB1L Are Required for Binding but Not for Accelerated Degradation of the Ubiquitin-like Modifier FAT10</dcterms:title> <dc:contributor>Schmidtke, Gunter</dc:contributor> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dc:contributor>Weber, Elvira</dc:contributor> <dc:contributor>Kalveram, Birte</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:abstract xml:lang="eng">Proteins selected for degradation are labeled with multiple molecules of ubiquitin and are subsequently cleaved by the 26 S proteasome. A family of proteins containing at least one ubiquitin-associated (UBA) domain and one ubiquitin-like (UBL) domain have been shown to act as soluble ubiquitin receptors of the 26 S proteasome and introduce a new level of specificity into the degradation system. They bind ubiquitylated proteins via their UBA domains and the 26 S proteasome via their UBL domain and facilitate the contact between substrate and protease. NEDD8 ultimate buster-1 long (NUB1L) belongs to this class of proteins and contains one UBL and three UBA domains.Werecently reported that NUB1L interacts with the ubiquitin-like modifier FAT10 and accelerates its degradation and that of its conjugates. Hereweshow that a deletion mutant of NUB1L lacking the UBL domain is still able to bind FAT10 but not the proteasome and no longer acceleratesFAT10degradation. Aversion ofNUB1Llacking all threeUBA domains, on the other hand, looses the ability to bind FAT10 but is still able to interact with the proteasome and accelerates the degradation of FAT10. Thedegradation of aFAT10mutantcontaining only the C-terminalUBLdomainis also still accelerated by NUB1L, even though the two proteins do not interact. In addition, we show that FAT10 and either one of its UBL domains alone can interact directly with the 26 S proteasome. We propose that NUB1L not only acts as a linker between the 26 S proteasome and ubiquitinlike proteins, but also as a facilitator of proteasomal degradation.</dcterms:abstract> <dc:creator>Schmidtke, Gunter</dc:creator> <dc:contributor>Bochtler, Petra</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-25T10:46:57Z</dc:date> <dc:language>eng</dc:language> <dc:creator>Bochtler, Petra</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-25T10:46:57Z</dcterms:available> <dc:creator>Lukasiak, Sebastian</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Hipp, Mark-Steffen</dc:creator> <dc:contributor>Lukasiak, Sebastian</dc:contributor> <dc:creator>Kalveram, Birte</dc:creator> <dcterms:bibliographicCitation>The Journal of Biological Chemistry ; 281 (2006), 29. - S. 20045-20054</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/22113"/> </rdf:Description> </rdf:RDF>

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