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Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand

Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand

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BECKMANN, Henning S. G., Andrea NIEDERWIESER, Manfred WIESSLER, Valentin WITTMANN, 2012. Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand. In: Chemistry - A European Journal. 18(21), pp. 6548-6554. ISSN 0947-6539. eISSN 1521-3765

@article{Beckmann2012-05-21Prepa-21830, title={Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand}, year={2012}, doi={10.1002/chem.201200382}, number={21}, volume={18}, issn={0947-6539}, journal={Chemistry - A European Journal}, pages={6548--6554}, author={Beckmann, Henning S. G. and Niederwieser, Andrea and Wiessler, Manfred and Wittmann, Valentin} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/21830"> <dcterms:title>Preparation of carbohydrate arrays by using Diels-Alder reactions with inverse electron demand</dcterms:title> <dc:creator>Wiessler, Manfred</dc:creator> <dc:contributor>Wittmann, Valentin</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Niederwieser, Andrea</dc:contributor> <dc:creator>Niederwieser, Andrea</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Wiessler, Manfred</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Wittmann, Valentin</dc:creator> <dcterms:bibliographicCitation>Chemistry : A European Journal ; 18 (2012), 21. - S. 6548-6554</dcterms:bibliographicCitation> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-18T10:02:20Z</dc:date> <dc:creator>Beckmann, Henning S. G.</dc:creator> <dcterms:abstract xml:lang="eng">Carbohydrate microarrays are an emerging tool for the high-throughput screening of carbohydrate–protein interactions that represent the basis of many biologically and medicinally relevant processes. The crucial step in the preparation of carbohydrate arrays is the attachment of carbohydrate probes to the surface. We examined the Diels–Alder reaction with inverse-electron-demand (DARinv) as an irreversible, chemoselective ligation reaction for that purpose. After having shown the efficiency of the DARinv in solution, we prepared a series of carbohydrate–dienophile conjugates that were printed onto tetrazine-modified glass slides. Binding experiments with fluorescently labeled lectins proved successful and homogeneous immobilization was achieved by the DARinv. For immobilization of nonfunctionalized reducing oligosaccharides we developed a bifunctional chemoselective linker that enabled the attachment of a dienophile tag to the oligosaccharides through oxime ligation. The conjugates obtained were successfully immobilized on glass slides. The presented strategies for the immobilization of both synthetic carbohydrate derivatives and unprotected reducing oligosaccharides facilitate the preparation of high-quality carbohydrate microarrays by means of the chemoselective DARinv. This concept can be readily adapted for the preparation of other biomolecule arrays.</dcterms:abstract> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21830"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-18T10:02:20Z</dcterms:available> <dcterms:issued>2012-05-21</dcterms:issued> <dc:contributor>Beckmann, Henning S. G.</dc:contributor> </rdf:Description> </rdf:RDF>

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