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Imaging of the DNA damage-induced dynamics of nuclear proteins via nonlinear photoperturbation

Imaging of the DNA damage-induced dynamics of nuclear proteins via nonlinear photoperturbation

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TOMAS, Martin, Philipp BLUMHARDT, Anja DEUTZMANN, Tobias SCHWARZ, Dimitri KROMM, Alfred LEITENSTORFER, Elisa FERRANDO-MAY, 2013. Imaging of the DNA damage-induced dynamics of nuclear proteins via nonlinear photoperturbation. In: Journal of Biophotonics. 6(8), pp. 645-655. ISSN 1864-063X. eISSN 1864-0648

@article{Tomas2013-08Imagi-21695, title={Imaging of the DNA damage-induced dynamics of nuclear proteins via nonlinear photoperturbation}, year={2013}, doi={10.1002/jbio.201200170}, number={8}, volume={6}, issn={1864-063X}, journal={Journal of Biophotonics}, pages={645--655}, author={Tomas, Martin and Blumhardt, Philipp and Deutzmann, Anja and Schwarz, Tobias and Kromm, Dimitri and Leitenstorfer, Alfred and Ferrando-May, Elisa} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/21695"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-16T12:11:23Z</dcterms:available> <dcterms:bibliographicCitation>Journal of Biophotonics ; 6 (2013), 8. - S. 645–655</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:abstract>Understanding the cellular response to DNA strand breaks is crucial to decipher the mechanisms maintaining the integrity of our genome. We present a novel method to visualize how the mobility of nuclear proteins changes in response to localized DNA damage. DNA strand breaks are induced via nonlinear excitation with femtosecond laser pulses at λ = 1050 nm in a 3D-confined subnuclear volume. After a time delay of choice, protein mobility within this volume is analysed by two-photon photoactivation of PA-GFP fusion proteins at λ = 775 nm. By changing the position of the photoactivation spot with respect to the zone of lesion the influence of chromatin structure and of the distance from damage are investigated. As first applications we demonstrate a locally confined, time-dependent mobility increase of histone H1.2, and a progressive retardation of the DNA repair factor XRCC1 at damaged sites. This assay can be used to map the response of nuclear proteins to DNA damage in time and space.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> <dc:creator>Deutzmann, Anja</dc:creator> <dc:creator>Tomas, Martin</dc:creator> <dc:creator>Ferrando-May, Elisa</dc:creator> <dc:contributor>Ferrando-May, Elisa</dc:contributor> <dc:creator>Blumhardt, Philipp</dc:creator> <dc:contributor>Schwarz, Tobias</dc:contributor> <dc:contributor>Deutzmann, Anja</dc:contributor> <dc:contributor>Leitenstorfer, Alfred</dc:contributor> <dc:contributor>Tomas, Martin</dc:contributor> <dc:creator>Leitenstorfer, Alfred</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-16T12:11:23Z</dc:date> <dc:contributor>Blumhardt, Philipp</dc:contributor> <dcterms:issued>2013-08</dcterms:issued> <dc:contributor>Kromm, Dimitri</dc:contributor> <dc:creator>Kromm, Dimitri</dc:creator> <dc:language>eng</dc:language> <dcterms:title>Imaging of the DNA damage-induced dynamics of nuclear proteins via nonlinear photoperturbation</dcterms:title> <dc:creator>Schwarz, Tobias</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21695"/> </rdf:Description> </rdf:RDF>

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