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Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum

Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum

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OEHLER, Dirk, Anja POEHLEIN, Andreas LEIMBACH, Nicolai MÜLLER, Rolf DANIEL, Gerhard GOTTSCHALK, Bernhard SCHINK, 2012. Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum. In: BMC Genomics. 13(1), 723. eISSN 1471-2164

@article{Oehler2012Genom-21413, title={Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum}, year={2012}, doi={10.1186/1471-2164-13-723}, number={1}, volume={13}, journal={BMC Genomics}, author={Oehler, Dirk and Poehlein, Anja and Leimbach, Andreas and Müller, Nicolai and Daniel, Rolf and Gottschalk, Gerhard and Schink, Bernhard}, note={Article Number: 723} }

Daniel, Rolf BMC Genomics ; 13 (2012). - 723 Gottschalk, Gerhard 2013-02-12T09:28:14Z Poehlein, Anja Müller, Nicolai Schink, Bernhard Gottschalk, Gerhard Leimbach, Andreas Background: Thermacetogenium phaeum is a thermophilic strictly anaerobic bacterium oxidizing acetate to CO2 in syntrophic association with a methanogenic partner. It can also grow in pure culture, e.g., by fermentation of<br />methanol to acetate. The key enzymes of homoacetate fermentation (Wood-Ljungdahl pathway) are used both in acetate oxidation and acetate formation. The obvious reversibility of this pathway in this organism is of specific<br />interest since syntrophic acetate oxidation operates close to the energetic limitations of microbial life.<br /><br /><br />Results: The genome of Th. phaeum is organized on a single circular chromosome and has a total size of 2,939,057 bp. It comprises 3.215 open reading frames of which 75% could be assigned to a gene function. The G+C content is 53.88 mol%. Many CRISPR sequences were found, indicating heavy phage attack in the past. A complete gene set<br />for a phage was found in the genome, and indications of phage action could also be observed in culture. The genome contained all genes required for CO2 reduction through the Wood-Ljungdahl pathway, including two formyl tetrahydrofolate ligases, three carbon monoxide dehydrogenases, one formate hydrogenlyase complex, three further formate dehydrogenases, and three further hydrogenases. The bacterium contains a menaquinone MQ-7. No indications of cytochromes or Rnf complexes could be found in the genome.<br /><br /><br />Conclusions: The information obtained from the genome sequence indicates that Th. phaeum differs basically from the three homoacetogenic bacteria sequenced so far, i.e., the sodium ion-dependent Acetobacterium woodii, the ethanol-producing Clostridium ljungdahlii, and the cytochrome-containing Moorella thermoacetica. The specific<br />enzyme outfit of Th. phaeum obviously allows ATP formation both in acetate formation and acetate oxidation. Schink, Bernhard Oehler, Dirk eng Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum Daniel, Rolf 2012 deposit-license Leimbach, Andreas Oehler, Dirk 2013-02-12T09:28:14Z Müller, Nicolai Poehlein, Anja

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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