Giant lipid vesicles under electric field pulses assessed by non invasive imaging

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MAUROY, Chloé, Thomas PORTET, Martin WINTERHALDER, Elisabeth BELLARD, Marie-Claire BLACHE, Justin TEISSIÉ, Andreas ZUMBUSCH, Marie-Pierre ROLS, 2012. Giant lipid vesicles under electric field pulses assessed by non invasive imaging. In: Bioelectrochemistry. 87, pp. 253-259. ISSN 1567-5394. eISSN 1878-562X. Available under: doi: 10.1016/j.bioelechem.2012.03.008

@article{Mauroy2012-10Giant-21122, title={Giant lipid vesicles under electric field pulses assessed by non invasive imaging}, year={2012}, doi={10.1016/j.bioelechem.2012.03.008}, volume={87}, issn={1567-5394}, journal={Bioelectrochemistry}, pages={253--259}, author={Mauroy, Chloé and Portet, Thomas and Winterhalder, Martin and Bellard, Elisabeth and Blache, Marie-Claire and Teissié, Justin and Zumbusch, Andreas and Rols, Marie-Pierre} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/21122"> <dcterms:abstract xml:lang="eng">We present experimental results regarding the effects of electric pulses on giant unilamellar vesicles (GUVs). We have used phase contrast and coherent anti-Stokes Raman scattering (CARS) microscopy as relevant optical approaches to gain insight into membrane changes under electropermeabilization. No addition of exogenous molecules (lipid analogue, fluorescent dye) was needed. Therefore, experiments were performed on pure lipid systems avoiding possible artefacts linked to their use. Structural membrane changes were assessed by loss of contrast inside the GUVs due to sucrose and glucose mixing. Our observations, performed at the single vesicle level, indicate these changes are under the control of the number of pulses and field intensity. Larger number of pulses enhances membrane alterations. A threshold value of the field intensity must be applied to allow exchange of molecules between GUVs and the external medium. This threshold depends on the size of the vesicles, the larger GUVs being affected at lower electric field strengths than the smaller ones. Our experimental data are well described by a simple model in which molecule entry is driven by direct exchange. The CARS microscopic study of the effect of pulse duration confirms that pulses, in the ms time range, induce loss of lipids and membrane deformations facing the electrodes.</dcterms:abstract> <dc:creator>Bellard, Elisabeth</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21122"/> <dc:contributor>Mauroy, Chloé</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21122/2/Mauroy_211222.pdf"/> <dc:contributor>Winterhalder, Martin</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21122/2/Mauroy_211222.pdf"/> <dc:creator>Teissié, Justin</dc:creator> <dc:contributor>Zumbusch, Andreas</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Winterhalder, Martin</dc:creator> <dc:contributor>Bellard, Elisabeth</dc:contributor> <dc:creator>Rols, Marie-Pierre</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-19T09:32:46Z</dc:date> <dc:creator>Mauroy, Chloé</dc:creator> <dc:contributor>Portet, Thomas</dc:contributor> <dc:language>eng</dc:language> <dcterms:title>Giant lipid vesicles under electric field pulses assessed by non invasive imaging</dcterms:title> <dc:rights>deposit-license</dc:rights> <dcterms:issued>2012-10</dcterms:issued> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Zumbusch, Andreas</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-19T09:32:46Z</dcterms:available> <dc:contributor>Teissié, Justin</dc:contributor> <dc:creator>Portet, Thomas</dc:creator> <dc:contributor>Rols, Marie-Pierre</dc:contributor> <dc:contributor>Blache, Marie-Claire</dc:contributor> <dc:creator>Blache, Marie-Claire</dc:creator> <dcterms:bibliographicCitation>Bioelectrochemistry ; 87 (2012). - S. 253-259</dcterms:bibliographicCitation> </rdf:Description> </rdf:RDF>

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