Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:cb12e3a235c2c5928c333538326ff0b6

WELTE, Wolfram, Monika LEONHARD, Kay DIEDERICHS, Hans-Ulrich WELTZIEN, Colin RESTALL, Christine HALL, Dennis CHAPMAN, 1989. Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol). In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 984(2), pp. 193-199. ISSN 0005-2736

@article{Welte1989Stabi-20875, title={Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)}, year={1989}, doi={10.1016/0005-2736(89)90216-2}, number={2}, volume={984}, issn={0005-2736}, journal={Biochimica et Biophysica Acta (BBA) - Biomembranes}, pages={193--199}, author={Welte, Wolfram and Leonhard, Monika and Diederichs, Kay and Weltzien, Hans-Ulrich and Restall, Colin and Hall, Christine and Chapman, Dennis} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/20875"> <dc:language>eng</dc:language> <dc:creator>Chapman, Dennis</dc:creator> <dc:creator>Hall, Christine</dc:creator> <dc:contributor>Welte, Wolfram</dc:contributor> <dc:contributor>Chapman, Dennis</dc:contributor> <dc:creator>Restall, Colin</dc:creator> <dcterms:title>Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)</dcterms:title> <dc:creator>Leonhard, Monika</dc:creator> <dc:contributor>Restall, Colin</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Diederichs, Kay</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-19T07:18:39Z</dc:date> <dcterms:abstract xml:lang="eng">The (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum (SR) has been solubilized with 1-alkanoyl propanediol 3-phosphorylcholines with chainlengths ranging between 8 and 12 C atoms. A marked dependence of the ATPase activity upon the chainlength was found, indicating that alkyl chainlengths with 12 C atoms are necessary for retention of activity. Addition of poly(ethylene glycol) to the eluting buffers used for gel filtration of the ATPase-detergent micelles was found to increase the activity and the long-term stability significantly. In the presence of Ca2+, the elution volume indicated an ATPase dimer, whereas in the absence of Ca2+ the elution volume indicated a monomeric solution. The purity of the preparations after gel filtration was improved by subsequent chromatography with a hydroxyapatite column.</dcterms:abstract> <dcterms:bibliographicCitation>Biochimica et Biophysica Acta (BBA) - Biomembranes ; 984 (1989), 2. - S. 193-199</dcterms:bibliographicCitation> <dc:creator>Diederichs, Kay</dc:creator> <dc:contributor>Hall, Christine</dc:contributor> <dc:creator>Weltzien, Hans-Ulrich</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20875"/> <dcterms:issued>1989</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:contributor>Leonhard, Monika</dc:contributor> <dc:contributor>Weltzien, Hans-Ulrich</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-19T07:18:39Z</dcterms:available> <dc:creator>Welte, Wolfram</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Welte_208757.pdf 3

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto