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(R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate : a patchwork pathway

(R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate : a patchwork pathway

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MAYER, Jutta, Karin DENGER, Klaus HOLLEMEYER, David SCHLEHECK, Alasdair M. COOK, 2012. (R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate : a patchwork pathway. In: Archives of Microbiology. 194(11), pp. 949-957. ISSN 0302-8933. eISSN 1432-072X

@article{Mayer2012-11(R)-C-20852, title={(R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate : a patchwork pathway}, year={2012}, doi={10.1007/s00203-012-0825-y}, number={11}, volume={194}, issn={0302-8933}, journal={Archives of Microbiology}, pages={949--957}, author={Mayer, Jutta and Denger, Karin and Hollemeyer, Klaus and Schleheck, David and Cook, Alasdair M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/20852"> <dc:contributor>Mayer, Jutta</dc:contributor> <dc:contributor>Hollemeyer, Klaus</dc:contributor> <dcterms:bibliographicCitation>Archives of Microbiology ; 194 (2012), 11. - S. 949-957</dcterms:bibliographicCitation> <dc:creator>Denger, Karin</dc:creator> <dcterms:abstract>Cupriavidus necator H16 grew exponentially with (R)-cysteate, a structural analogue of aspartate, as sole source of nitrogen in succinate-salts medium. Utilization of cysteate was quantitative and concomitant with growth and with the excretion of the deaminated product (R)-sulfolactate, which was identified thoroughly. The deaminative pathway started with transport of (R)-cysteate into the cell, which we attributed to an aspartate transporter. Transamination to sulfopyruvate involved an aspartate/(R)-cysteate:2-oxoglutarate aminotransferase (Aoa/Coa) and regeneration of the amino group acceptor by NADP⁺-coupled glutamate dehydrogenase. Reduction of sulfopyruvate to (R)-sulfolactate was catalyzed by a (S)-malate/(R)-sulfolactate dehydrogenase (Mdh/Sdh). Excretion of the sulfolactate could be attributed to the sulfite/organosulfonate exporter TauE, which was co-encoded and co-expressed, with sulfoacetaldehyde acetyltransferase (Xsc), though Xsc was irrelevant to the current pathway. The metabolic enzymes could be assayed biochemically. Aoa/Coa and Mdh/Sdh were highly enriched by protein separation, partly characterized, and the relevant locus-tags identified by peptide-mass fingerprinting. Finally, RT-PCR was used to confirm the transcription of all appropriate genes. We thus demonstrated that Cupriavidus necator H16 uses a patchwork pathway by recruitment of 'housekeeping' genes and sulfoacetaldehyde-degradative genes to scavenge for (R)-cysteate-nitrogen.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-16T07:43:20Z</dc:date> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:creator>Mayer, Jutta</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:contributor>Schleheck, David</dc:contributor> <dcterms:issued>2012-11</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-16T07:43:20Z</dcterms:available> <dcterms:title>(R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate : a patchwork pathway</dcterms:title> <dc:creator>Schleheck, David</dc:creator> <dc:contributor>Denger, Karin</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20852"/> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Hollemeyer, Klaus</dc:creator> </rdf:Description> </rdf:RDF>

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