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Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines

Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines

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HALDER, Rashi, Jean-Francois RIOU, Marie-Paule TEULADE-FICHOU, Tancred FRICKEY, Jörg S. HARTIG, 2012. Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines. In: BMC Research Notes. 5(1), 138. ISSN 1756-0500. eISSN 1756-0500

@article{Halder2012Bisqu-20596, title={Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines}, year={2012}, doi={10.1186/1756-0500-5-138}, number={1}, volume={5}, issn={1756-0500}, journal={BMC Research Notes}, author={Halder, Rashi and Riou, Jean-Francois and Teulade-Fichou, Marie-Paule and Frickey, Tancred and Hartig, Jörg S.}, note={Article Number: 138} }

Frickey, Tancred 2012-10-05T08:42:13Z Riou, Jean-Francois 2012 Hartig, Jörg S. Frickey, Tancred Halder, Rashi Hartig, Jörg S. Teulade-Fichou, Marie-Paule Halder, Rashi deposit-license Bisquinolinium compounds induce quadruplex-specific transcriptome changes in HeLa S3 cell lines Teulade-Fichou, Marie-Paule BMC Research Notes ; 5 (2012). - 138 eng Background<br /><br /><br /><br />Guanosine rich sequences capable of forming G-quadruplex (G4) motifs are enriched near the gene transcription start site (TSS) in the human genome. When probed at the single gene level, G-quadruplex motifs residing in promoter regions show substantial effects on gene transcription. Moreover, further changes in transcription levels are noticed when G4-motifs are targeted with G-quadruplex-specific small molecules.<br /><br />Results<br /><br /><br /><br />Global studies concerning general changes of the transcriptome via targeting promoter-based G-quadruplex motifs are very limited and have so far only been carried out with compounds displaying weak selectivity for quadruplex sequences. Here we utilize two G-quadruplex-specific bisquinolinium derivatives PhenDC3 and 360A and investigate their effects on the expression of the HeLa S3 transcriptome. Our results show wide-spread changes in the transcriptome with specificity for genes with G-quadruplex motifs near their transcription start sites (TSS). Using real-time PCR we further confirmed the specificity of PhenDC3 and 360A as potent molecules to target G-quadruplex-regulated genes.<br /><br />Conclusions<br /><br /><br /><br />Specific effects on quadruplex-containing genes have been observed utilizing whole-transcriptome analysis upon treatment of cultured cells with quadruplex-selective bisquinolinium compounds. 2012-10-05T08:42:13Z Riou, Jean-Francois

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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