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Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides

Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides

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SIEGMUND, Vanessa, Tobias SANTNER, Ronald MICURA, Andreas MARX, 2012. Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides. In: Chemical Communications. 48(79), pp. 9870-9872. ISSN 1359-7345. eISSN 1364-548X

@article{Siegmund2012-10-11Scree-20581, title={Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides}, year={2012}, doi={10.1039/C2CC35028A}, number={79}, volume={48}, issn={1359-7345}, journal={Chemical Communications}, pages={9870--9872}, author={Siegmund, Vanessa and Santner, Tobias and Micura, Ronald and Marx, Andreas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/20581"> <dc:creator>Micura, Ronald</dc:creator> <dcterms:title>Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides</dcterms:title> <dc:contributor>Siegmund, Vanessa</dc:contributor> <dc:language>eng</dc:language> <dc:rights>deposit-license</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-07-31T22:25:03Z</dcterms:available> <dc:contributor>Micura, Ronald</dc:contributor> <dcterms:issued>2012-10-11</dcterms:issued> <dc:creator>Siegmund, Vanessa</dc:creator> <dcterms:abstract xml:lang="eng">We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.</dcterms:abstract> <dc:creator>Marx, Andreas</dc:creator> <dcterms:bibliographicCitation>Chemical communications : ChemComm ; 48 (2012), 79. - S. 9870-9872</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20581"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-07T10:14:30Z</dc:date> <dc:contributor>Marx, Andreas</dc:contributor> <dc:contributor>Santner, Tobias</dc:contributor> <dc:creator>Santner, Tobias</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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