KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Betz, Karin; Malyshev, Denis A.; Lavergne, Thomas; Welte, Wolfram; Diederichs, Kay; Dwyer, Tammy J.; Ordoukhanian, Phillip; Romesberg, Floyd E.; Marx, Andreas

KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Files

Betz_KlenTaq.pdf(598.91 KB)

Date

2012

Authors

Betz, Karin

Malyshev, Denis A.

Lavergne, Thomas

Welte, Wolfram

Diederichs, Kay

Dwyer, Tammy J.

Ordoukhanian, Phillip

Romesberg, Floyd E.

Marx, Andreas

Publication type

Journal article

Published in

Nature Chemical Biology ; 8 (2012), 7. - pp. 612-614. - ISSN 1552-4450. - eISSN 1552-4469

Abstract

Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair,dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

BETZ, Karin, Denis A. MALYSHEV, Thomas LAVERGNE, Wolfram WELTE, Kay DIEDERICHS, Tammy J. DWYER, Phillip ORDOUKHANIAN, Floyd E. ROMESBERG, Andreas MARX, 2012. KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry. In: Nature Chemical Biology. 8(7), pp. 612-614. ISSN 1552-4450. eISSN 1552-4469. Available under: doi: 10.1038/nchembio.966

BibTex

@article{Betz2012-07KlenT-19699,
  year={2012},
  doi={10.1038/nchembio.966},
  title={KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry},
  number={7},
  volume={8},
  issn={1552-4450},
  journal={Nature Chemical Biology},
  pages={612--614},
  author={Betz, Karin and Malyshev, Denis A. and Lavergne, Thomas and Welte, Wolfram and Diederichs, Kay and Dwyer, Tammy J. and Ordoukhanian, Phillip and Romesberg, Floyd E. and Marx, Andreas}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/19699">
    <dcterms:title>KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry</dcterms:title>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-06T23:25:05Z</dcterms:available>
    <dc:creator>Dwyer, Tammy J.</dc:creator>
    <dc:contributor>Betz, Karin</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Malyshev, Denis A.</dc:creator>
    <dc:creator>Romesberg, Floyd E.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair,dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.</dcterms:abstract>
    <dc:language>eng</dc:language>
    <dcterms:issued>2012-07</dcterms:issued>
    <dc:contributor>Ordoukhanian, Phillip</dc:contributor>
    <dc:creator>Ordoukhanian, Phillip</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19699/1/Betz_KlenTaq.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/19699"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Lavergne, Thomas</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: Nature Chemical Biology ; 8 (2012), 7. - pp. 612-614</dcterms:bibliographicCitation>
    <dc:creator>Lavergne, Thomas</dc:creator>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:contributor>Malyshev, Denis A.</dc:contributor>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-07-05T06:01:55Z</dc:date>
    <dc:contributor>Romesberg, Floyd E.</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Dwyer, Tammy J.</dc:contributor>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:creator>Betz, Karin</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19699/1/Betz_KlenTaq.pdf"/>
  </rdf:Description>
</rdf:RDF>

Bibliography of Konstanz

Yes