KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-196996 |
| Author: | Betz, Karin; Malyshev, Denis A.; Lavergne, Thomas; Welte, Wolfram; Diederichs, Kay; Dwyer, Tammy J.; Ordoukhanian, Phillip; Romesberg, Floyd E.; Marx, Andreas |
| Year of publication: | 2012 |
| Published in: | Nature Chemical Biology ; 8 (2012), 7. - pp. 612-614. - ISSN 1552-4450. - eISSN 1552-4469 |
| Pubmed ID: | 22660438 |
| DOI (citable link): | https://dx.doi.org/10.1038/nchembio.966 |
| Summary: |
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair,dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
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BETZ, Karin, Denis A. MALYSHEV, Thomas LAVERGNE, Wolfram WELTE, Kay DIEDERICHS, Tammy J. DWYER, Phillip ORDOUKHANIAN, Floyd E. ROMESBERG, Andreas MARX, 2012. KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry. In: Nature Chemical Biology. 8(7), pp. 612-614. ISSN 1552-4450. eISSN 1552-4469. Available under: doi: 10.1038/nchembio.966
@article{Betz2012-07KlenT-19699, title={KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry}, year={2012}, doi={10.1038/nchembio.966}, number={7}, volume={8}, issn={1552-4450}, journal={Nature Chemical Biology}, pages={612--614}, author={Betz, Karin and Malyshev, Denis A. and Lavergne, Thomas and Welte, Wolfram and Diederichs, Kay and Dwyer, Tammy J. and Ordoukhanian, Phillip and Romesberg, Floyd E. and Marx, Andreas} }
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