Synthesis and characterization of a nonapeptides with specific properties for Cu binding
| Type of Publication: | Journal article |
| Author: | Drochioiu, Gabi; Murariu, Manuela; Petre, Brindusa; Manea, Marilena; Przybylski, Michael |
| Year of publication: | 2007 |
| Published in: | Revista de chimie : (The Chemistry Magazine) ; 58 (2007), 3. - pp. 311-315 |
| Summary: |
A nonapeptide with the following sequence of amino acid residues CHQYHHNRE has been synthesized and characterized. The peptide is useful to investigate the binding of copper ions to histidine-containing peptides and proteins. It was synthesised manually by Solid Phase Peptide Synthesis (SPPS) using Fmoc protection strategy. The peptide was subjected to final purification by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) and characterised by matrix assisted laser desorption ionization with time of flight mass spectrometry (MALDI-TOF MS). The GPMAW 6.11 (General Protein/Mass Analysis for Window) program was used for theoretical characterization of the peptide.
|
| Subject (DDC): | 540 Chemistry |
| Keywords: | peptide synthesis, Fmoc strategy, mass spectrometry, copper binding |
| Bibliography of Konstanz: | Yes |
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DROCHIOIU, Gabi, Manuela MURARIU, Brindusa PETRE, Marilena MANEA, Michael PRZYBYLSKI, 2007. Synthesis and characterization of a nonapeptides with specific properties for Cu binding. In: Revista de chimie : (The Chemistry Magazine). 58(3), pp. 311-315
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