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The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells

The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells

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ROSAZZA, Christelle, Jean-Michel ESCOFFRE, Andreas ZUMBUSCH, Marie-Pierre ROLS, 2011. The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells. In: Molecular Therapy. 19(5), pp. 913-921. ISSN 1525-0016. eISSN 1525-0024

@article{Rosazza2011-05actin-19303, title={The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells}, year={2011}, doi={10.1038/mt.2010.303}, number={5}, volume={19}, issn={1525-0016}, journal={Molecular Therapy}, pages={913--921}, author={Rosazza, Christelle and Escoffre, Jean-Michel and Zumbusch, Andreas and Rols, Marie-Pierre} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/19303"> <dcterms:title>The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells</dcterms:title> <dc:creator>Rosazza, Christelle</dc:creator> <dc:contributor>Escoffre, Jean-Michel</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-05-16T06:31:41Z</dcterms:available> <dcterms:issued>2011-05</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:creator>Rols, Marie-Pierre</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-05-16T06:31:41Z</dc:date> <dc:contributor>Rosazza, Christelle</dc:contributor> <dcterms:abstract xml:lang="eng">Electrotransfer of molecules is a well established technique which finds extensive use for gene transfer and holds great promise for anticancer treatment. Despite its widespread application, the mechanisms governing the entry of DNA into the cell and its intracellular trafficking are not yet known. The aim of this study is to unravel the role of the actin cytoskeleton during gene electrotransfer in cells. We performed single-cell level approaches to observe the organization of the actin cytoskeleton in Chinese hamster ovary (CHO) cells. In addition, we performed experiments at the multiple-cell level to evaluate the efficiency of DNA transfer after alteration of the actin cytoskeleton using the drug latrunculin B. Actin patches colocalizing with the DNA at the plasma membrane were observed with additional characteristics similar to those of the DNA aggregates in terms of time, number, and size. The disruption of the microfilaments reduces the DNA accumulation at the plasma membrane and the gene expression. This is the first direct experimental evidence of the participation of the actin cytoskeleton in DNA electrotransfer.</dcterms:abstract> <dc:language>eng</dc:language> <dc:creator>Zumbusch, Andreas</dc:creator> <dc:creator>Escoffre, Jean-Michel</dc:creator> <dcterms:bibliographicCitation>Molecular therapy ; 19 (2011), 5. - S. 913-921</dcterms:bibliographicCitation> <dc:contributor>Rols, Marie-Pierre</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/19303"/> <dc:contributor>Zumbusch, Andreas</dc:contributor> </rdf:Description> </rdf:RDF>

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