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Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes

Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes

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HOLZBERGER, Bastian, Julian STROHMEIER, Vanessa SIEGMUND, Ulf DIEDERICHSEN, Andreas MARX, 2012. Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes. In: Bioorganic & Medicinal Chemistry Letters. 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405

@article{Holzberger2012-05-01Enzym-19279, title={Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes}, year={2012}, doi={10.1016/j.bmcl.2012.03.056}, number={9}, volume={22}, issn={0960-894X}, journal={Bioorganic & Medicinal Chemistry Letters}, pages={3136--3139}, author={Holzberger, Bastian and Strohmeier, Julian and Siegmund, Vanessa and Diederichsen, Ulf and Marx, Andreas} }

Strohmeier, Julian 2012-05-01 2012-05-10T09:43:11Z Holzberger, Bastian First publ. in: Bioorganic & Medicinal Chemistry Letters ; 22 (2012), 9. - pp. 3136-3139 Marx, Andreas Siegmund, Vanessa 2012-05-10T09:43:11Z deposit-license Siegmund, Vanessa Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes Fluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2′-deoxyguanosine were synthesized and converted into the corresponding 5′-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2′-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy. Diederichsen, Ulf Holzberger, Bastian Strohmeier, Julian eng Diederichsen, Ulf Marx, Andreas

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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