Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes

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2012
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Strohmeier, Julian
Diederichsen, Ulf
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Bioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056
Zusammenfassung

Fluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2′-deoxyguanosine were synthesized and converted into the corresponding 5′-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2′-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy.

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540 Chemie
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DNA, Modified nucleotide, DNA polymerase, Fluorescent analog, Fluorescence spectroscopy
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ISO 690HOLZBERGER, Bastian, Julian STROHMEIER, Vanessa SIEGMUND, Ulf DIEDERICHSEN, Andreas MARX, 2012. Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes. In: Bioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056
BibTex
@article{Holzberger2012-05-01Enzym-19279,
  year={2012},
  doi={10.1016/j.bmcl.2012.03.056},
  title={Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes},
  number={9},
  volume={22},
  issn={0960-894X},
  journal={Bioorganic & Medicinal Chemistry Letters},
  pages={3136--3139},
  author={Holzberger, Bastian and Strohmeier, Julian and Siegmund, Vanessa and Diederichsen, Ulf and Marx, Andreas}
}
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