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Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes

Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes

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GALETSKIY, Dmitry, Jens LOHSCHEIDER, Alexey S. KONONIKHIN, Igor A. POPOV, Eugene N. NIKOLAEV, Iwona ADAMSKA, 2011. Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes. In: Rapid Communications in Mass Spectrometry. 25(1), pp. 184-190. ISSN 0951-4198. eISSN 1097-0231

@article{Galetskiy2011-01-15spect-18974, title={Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes}, year={2011}, doi={10.1002/rcm.4855}, number={1}, volume={25}, issn={0951-4198}, journal={Rapid Communications in Mass Spectrometry}, pages={184--190}, author={Galetskiy, Dmitry and Lohscheider, Jens and Kononikhin, Alexey S. and Popov, Igor A. and Nikolaev, Eugene N. and Adamska, Iwona} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/18974"> <dc:contributor>Kononikhin, Alexey S.</dc:contributor> <dc:contributor>Adamska, Iwona</dc:contributor> <dc:creator>Lohscheider, Jens</dc:creator> <dc:contributor>Popov, Igor A.</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Rapid Communications in Mass Spectrometry ; 25 (2011), 1. - pp. 184-190</dcterms:bibliographicCitation> <dcterms:title>Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes</dcterms:title> <dcterms:abstract xml:lang="eng">Oxidative and nitrosative stress leaves footprints in the plant chloroplast in the form of oxidatively modified proteins. Using a mass spectrometric approach, we identified 126 tyrosine and 12 tryptophan nitration sites in 164 nitrated proteolytic peptides, mainly from photosystem I (PSI), photosystem II (PSII), cytochrome b6/f and ATP-synthase complexes and 140 oxidation products of tyrosine, tryptophan, proline, phenylalanine and histidine residues. While a high number of nitration sites were found in proteins from four photosynthetic complexes indicating that the nitration belongs to one of the prominent posttranslational protein modifications in photosynthetic apparatus, amino acid oxidation products were determined mostly in PSII and to a lower extent in PSI. Exposure of plants to light stress resulted in an increased level of tyrosine and tryptophan nitration and tryptophan oxidation in proteins of PSII reaction center and the oxygen-evolving complex, as compared to low light conditions. In contrast, the level of nitration and oxidation of these amino acid residues strongly decreased for all light-harvesting proteins of PSII under the same conditions. Based on these data, we propose that oxidative modifications of proteins by reactive oxygen and nitrogen species might represent an important regulatory mechanism of protein turnover under light stress conditions, especially for PSII and its antenna proteins.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/18974"/> <dc:creator>Popov, Igor A.</dc:creator> <dc:creator>Nikolaev, Eugene N.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-04-12T05:41:55Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-04-12T05:41:55Z</dc:date> <dc:creator>Kononikhin, Alexey S.</dc:creator> <dc:contributor>Lohscheider, Jens</dc:contributor> <dcterms:issued>2011-01-15</dcterms:issued> <dc:creator>Galetskiy, Dmitry</dc:creator> <dc:contributor>Galetskiy, Dmitry</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:language>eng</dc:language> <dc:creator>Adamska, Iwona</dc:creator> <dc:contributor>Nikolaev, Eugene N.</dc:contributor> </rdf:Description> </rdf:RDF>

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