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Toward bioinspired galectin mimetics : Identification of ligand-contacting peptides by proteolytic-excision mass spectrometry

Toward bioinspired galectin mimetics : Identification of ligand-contacting peptides by proteolytic-excision mass spectrometry

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Prüfsumme: MD5:54a6fc7040b3366e6c8dfd1256068a32

MOISE, Adrian, Sabine ANDRÉ, Frederike EGGERS, Mickael KRZEMINSKI, Michael PRZYBYLSKI, Hans-Joachim GABIUS, 2011. Toward bioinspired galectin mimetics : Identification of ligand-contacting peptides by proteolytic-excision mass spectrometry. In: Journal of the American Chemical Society. 133(38), pp. 14844-14847. ISSN 0002-7863. eISSN 1520-5126

@article{Moise2011-09-28Towar-18625, title={Toward bioinspired galectin mimetics : Identification of ligand-contacting peptides by proteolytic-excision mass spectrometry}, year={2011}, doi={10.1021/ja201967v}, number={38}, volume={133}, issn={0002-7863}, journal={Journal of the American Chemical Society}, pages={14844--14847}, author={Moise, Adrian and André, Sabine and Eggers, Frederike and Krzeminski, Mickael and Przybylski, Michael and Gabius, Hans-Joachim} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/18625"> <dc:creator>Gabius, Hans-Joachim</dc:creator> <dc:contributor>Moise, Adrian</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Przybylski, Michael</dc:creator> <dc:language>eng</dc:language> <dc:creator>André, Sabine</dc:creator> <dc:contributor>André, Sabine</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-03-19T11:50:17Z</dcterms:available> <dc:creator>Krzeminski, Mickael</dc:creator> <dcterms:abstract xml:lang="eng">Clinically relevant bioactivities of human galectins (adhesion/growth-regulatory galactoside-specific lectins) inspired the design of peptides as new tools to elicit favorable effects (e.g., in growth control) or block harmful binding (e.g., in tissue invasion). To obtain the bioinspired lead compounds, we combined a proteolytic fragmentation approach without/with ligand contact (excision) with mass spectrometric identification of affinity-bound protein fragments, using galectin-1 and -3 as models. Two peptides from the carbohydrate recognition domains were obtained in each case in experimental series rigorously controlled for specificity, and the [157 162] peptide of galectin-3 proved to be active in blocking lectin binding to a neoglycoprotein and to tumor cell surfaces. This approach affords peptide sequences for structural optimization and intrafamily/phylogenetic galectin comparison at the binding-site level with a minimal requirement of protein quantity, and it is even amenable to mixtures.</dcterms:abstract> <dcterms:issued>2011-09-28</dcterms:issued> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:creator>Moise, Adrian</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of the American Chemical Society ; 133 (2011), 38. - pp. 14844-14847</dcterms:bibliographicCitation> <dc:creator>Eggers, Frederike</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-03-19T11:50:17Z</dc:date> <dc:contributor>Eggers, Frederike</dc:contributor> <dcterms:title>Toward bioinspired galectin mimetics : Identification of ligand-contacting peptides by proteolytic-excision mass spectrometry</dcterms:title> <dc:contributor>Gabius, Hans-Joachim</dc:contributor> <dc:rights>deposit-license</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/18625"/> <dc:contributor>Krzeminski, Mickael</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Przybylski etal.pdf 219

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