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Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes

Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes

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AZARKH, Mykhailo, Oliver OKLE, Philipp EYRING, Daniel DIETRICH, Malte DRESCHER, 2011. Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes. In: Journal of Magnetic Resonance. 212(2), pp. 450-454. ISSN 1090-7807. eISSN 1096-0856

@article{Azarkh2011-10Evalu-18165, title={Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes}, year={2011}, doi={10.1016/j.jmr.2011.07.014}, number={2}, volume={212}, issn={1090-7807}, journal={Journal of Magnetic Resonance}, pages={450--454}, author={Azarkh, Mykhailo and Okle, Oliver and Eyring, Philipp and Dietrich, Daniel and Drescher, Malte} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/18165"> <dc:contributor>Eyring, Philipp</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/18165"/> <dc:creator>Azarkh, Mykhailo</dc:creator> <dc:creator>Drescher, Malte</dc:creator> <dc:creator>Dietrich, Daniel</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Magnetic Resonance ; 212 (2011), 2. - pp. 450–454</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-02-14T07:38:44Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-02-14T07:38:44Z</dc:date> <dc:language>eng</dc:language> <dc:contributor>Okle, Oliver</dc:contributor> <dc:creator>Okle, Oliver</dc:creator> <dc:contributor>Azarkh, Mykhailo</dc:contributor> <dcterms:issued>2011-10</dcterms:issued> <dcterms:abstract xml:lang="eng">Spin-label electron paramagnetic resonance (SL-EPR) spectroscopy has become a powerful and useful tool for studying structure and dynamics of biomacromolecules. However, utilizing these methods at physiological temperatures for in-cell studies is hampered by reduction of the nitroxide spin labels and thus short half-lives in the cellular environment. Consequently, reduction kinetics of two structurally different nitroxides was investigated in cell extracts of Xenopus laevis oocytes using rapid-scan cw-exper- iments at X-band. The five member heterocyclic ring nitroxide PCA (3-carboxy-2,2,5,5-tetramethylpyrro- lidinyl-1-oxy) under investigation features much higher stability against intracellular reduction than the six member ring analog TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxilic acid) and is therefore a suitable spin label type for in-cell EPR. The kinetic data can be described according to the Michaelis–Menten model and thus suggest an enzymatic or enzyme-mediated reduction process.</dcterms:abstract> <dc:contributor>Dietrich, Daniel</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:title>Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes</dcterms:title> <dc:contributor>Drescher, Malte</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:creator>Eyring, Philipp</dc:creator> </rdf:Description> </rdf:RDF>

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