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The Automated FADU-Assay, a Potential High-Throughput In Vitro Method for Early Screening of DNA Breakage

The Automated FADU-Assay, a Potential High-Throughput In Vitro Method for Early Screening of DNA Breakage

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MORENO-VILLANUEVA, Maria, Tobias ELTZE, Dirk DRESSLER, Jürgen BERNHARDT, Cordula HIRSCH, Peter WICK, Gudrun VON SCHEVEN, Kirsten LEX, Alexander BÜRKLE, 2011. The Automated FADU-Assay, a Potential High-Throughput In Vitro Method for Early Screening of DNA Breakage. In: Alternatives to animal experimentation : ALTEX. 28(4), pp. 295-303. ISSN 1868-596X

@article{Moreno-Villanueva2011Autom-18028, title={The Automated FADU-Assay, a Potential High-Throughput In Vitro Method for Early Screening of DNA Breakage}, year={2011}, doi={10.14573/altex.2011.4.295}, number={4}, volume={28}, issn={1868-596X}, journal={Alternatives to animal experimentation : ALTEX}, pages={295--303}, author={Moreno-Villanueva, Maria and Eltze, Tobias and Dressler, Dirk and Bernhardt, Jürgen and Hirsch, Cordula and Wick, Peter and von Scheven, Gudrun and Lex, Kirsten and Bürkle, Alexander} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/18028"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/18028"/> <dc:contributor>Bernhardt, Jürgen</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Moreno-Villanueva, Maria</dc:creator> <dc:creator>Lex, Kirsten</dc:creator> <dc:contributor>Moreno-Villanueva, Maria</dc:contributor> <dc:contributor>von Scheven, Gudrun</dc:contributor> <dcterms:abstract xml:lang="eng">Genotoxicity tests are essential to identify compounds that have a potential to compromise not only the environment but also human and animal health, including compounds that increase the risk of cancer. At present, no single test is capable of detecting all types of genotoxic effects; therefore a battery of in vitro and, if positive, in vivo tests is necessary to determine the genotoxicity of a substance. However, the respective specificities of current assays have been criticized for their high percentage of false positive results. We recently published an automated version of the “Fluorimetric detection of Alkaline DNA Unwinding” (FADU) assay for measuring DNA strand breaks in human peripheral blood mononuclear cells or in cell lines. Using this new technology we show detection of DNA strand breaks in cells treated with several compounds known to induce DNA strand breaks by various mechanisms. We also tested toxic compounds that were not expected to induce DNA strand breaks; these were negative in the assay as expected. Finally, we included zinc oxide nanoparticles of high production volume to explore further fields of potential FADU applications. The main advantages of this assay are high reproducibility, easy handling, lack of operator bias, high-throughput, speed, and low cost.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-02-07T09:50:59Z</dc:date> <dc:creator>Bernhardt, Jürgen</dc:creator> <dc:creator>von Scheven, Gudrun</dc:creator> <dcterms:title>The Automated FADU-Assay, a Potential High-Throughput In Vitro Method for Early Screening of DNA Breakage</dcterms:title> <dc:contributor>Bürkle, Alexander</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Alternatives to animal experimentation : ALTEX ; 28 (2011), 4. - S. 295-303</dcterms:bibliographicCitation> <dc:creator>Hirsch, Cordula</dc:creator> <dc:creator>Wick, Peter</dc:creator> <dcterms:issued>2011</dcterms:issued> <dc:contributor>Wick, Peter</dc:contributor> <dc:contributor>Lex, Kirsten</dc:contributor> <dc:creator>Eltze, Tobias</dc:creator> <dc:creator>Dressler, Dirk</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-02-07T09:50:59Z</dcterms:available> <dc:contributor>Hirsch, Cordula</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Dressler, Dirk</dc:contributor> <dc:creator>Bürkle, Alexander</dc:creator> <dc:contributor>Eltze, Tobias</dc:contributor> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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