Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies

Zirah, Séverine; Stefanescu, Raluca; Manea, Marilena; Tian, Xiaodan; Cecal, Roxana; Kozin, Sergey A.; Debey, Pascale; Rebuffat, Sylvie; Przybylski, Michael

Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies

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Date

2004

Authors

Zirah, Séverine

Stefanescu, Raluca

Manea, Marilena

Tian, Xiaodan

Cecal, Roxana

Kozin, Sergey A.

Debey, Pascale

Rebuffat, Sylvie

Przybylski, Michael

Publication type

Journal article

Published in

Biochemical and Biophysical Research Communications ; 321 (2004), 2. - pp. 324-328. - ISSN 0006-291X

Abstract

Amyloid plaques associated to Alzheimer’s disease present a high content of zinc ions. We previously showed that the N-terminal region of the amyloid peptide Aβ constitutes an autonomous zinc-binding domain. This region encompasses the previously identified epitope Aβ(4–10) targeted by antibodies capable to reduce amyloid deposition, but the influence of Aβ/Zn binding on the epitope recognition remains unknown. We demonstrate here the effect of Zn²⁺ ions on the recognition of peptides sharing the sequence of the Aβ N-terminal domain, by two monoclonal antibodies recognizing the β-amyloid(4–10) epitope. The presence of Zn²⁺, but not of other cations, increased the recognition of the (1–16) peptide, while it was without effect on the recognition of the (1–10) peptide. These findings show a zinc-induced conformational change of the (1–16)-N-terminal region of Aβ, which results in a better accessibility of the Aβ(4–10) epitope to the anti-Aβ antibodies, and suggest a role of zinc in epitope-based vaccination approaches.

Subject (DDC)

540 Chemistry

Keywords

Alzheimer’s disease,β-Amyloid peptide,Zinc binding,Epitope

Cite This

ISO 690

ZIRAH, Séverine, Raluca STEFANESCU, Marilena MANEA, Xiaodan TIAN, Roxana CECAL, Sergey A. KOZIN, Pascale DEBEY, Sylvie REBUFFAT, Michael PRZYBYLSKI, 2004. Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies. In: Biochemical and Biophysical Research Communications. 321(2), pp. 324-328. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2004.06.150

BibTex

@article{Zirah2004-08-20bindi-17609,
  year={2004},
  doi={10.1016/j.bbrc.2004.06.150},
  title={Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies},
  number={2},
  volume={321},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={324--328},
  author={Zirah, Séverine and Stefanescu, Raluca and Manea, Marilena and Tian, Xiaodan and Cecal, Roxana and Kozin, Sergey A. and Debey, Pascale and Rebuffat, Sylvie and Przybylski, Michael}
}

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Bibliography of Konstanz

Yes