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T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining

T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining

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MICHEL, Nico, Peter ÖHLSCHLÄGER, Wolfram OSEN, Eva-Jasmin FREYSCHMIDT, Heidrun GUTHÖHRLEIN, Andreas M. KAUFMANN, Martin MÜLLER, Lutz GISSMANN, 2002. T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining. In: Intervirology. 45(4-6), pp. 290-299. ISSN 1423-0100. Available under: doi: 10.1159/000067923

@article{Michel2002respo-17505, title={T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining}, year={2002}, doi={10.1159/000067923}, number={4-6}, volume={45}, issn={1423-0100}, journal={Intervirology}, pages={290--299}, author={Michel, Nico and Öhlschläger, Peter and Osen, Wolfram and Freyschmidt, Eva-Jasmin and Guthöhrlein, Heidrun and Kaufmann, Andreas M. and Müller, Martin and Gissmann, Lutz} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/17505"> <dc:contributor>Öhlschläger, Peter</dc:contributor> <dc:creator>Kaufmann, Andreas M.</dc:creator> <dc:contributor>Kaufmann, Andreas M.</dc:contributor> <dc:creator>Freyschmidt, Eva-Jasmin</dc:creator> <dc:contributor>Michel, Nico</dc:contributor> <dc:language>eng</dc:language> <dc:rights>terms-of-use</dc:rights> <dc:creator>Müller, Martin</dc:creator> <dc:creator>Michel, Nico</dc:creator> <dc:contributor>Müller, Martin</dc:contributor> <dc:contributor>Freyschmidt, Eva-Jasmin</dc:contributor> <dc:creator>Öhlschläger, Peter</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-18T11:33:47Z</dcterms:available> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Osen, Wolfram</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Gissmann, Lutz</dc:creator> <dc:contributor>Guthöhrlein, Heidrun</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Intervirology ; 45 (2002), 4-6. - pp. 290-299</dcterms:bibliographicCitation> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Gissmann, Lutz</dc:contributor> <dcterms:issued>2002</dcterms:issued> <dc:creator>Guthöhrlein, Heidrun</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/17505"/> <dcterms:abstract xml:lang="eng">Successful vaccination against infections by high-risk papillomaviruses aiming at the prevention of cervical cancer most likely requires the induction of neutralizing antibodies and human papillomavirus (HPV)-specific T cells directed against early viral proteins such as E7. Whereas the technology for detection of antibodies is well established, measurement of T cells is more cumbersome and standardization of assays is difficult. By using chromium release assay, ELISPOT, tetramer staining and intracellular IFN-gamma assay, we compared the levels of HPV 16 E7-specific T cells obtained after immunization of C57BL/6 mice with different DNA expression vectors. We found that all four assays gave highly comparable results. ELISPOT can be recommended for future studies as it indicates the presence of activated (i.e. IFN-gamma-secreting) T cells in a quantitative manner and combines high sensitivity with relatively low T cell demand.</dcterms:abstract> <dcterms:title>T cell response to human papillomavirus 16 E7 in mice : comparison of Cr release assay, intracellular IFN-g=y [gamma] production, ELISPOT and tetramer staining</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-18T11:33:47Z</dc:date> <dc:creator>Osen, Wolfram</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> </rdf:Description> </rdf:RDF>

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