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Human Papillomavirus Type 16 L1 Capsomeres Induce L1-Specific Cytotoxic T Lymphocytes and Tumor Regression in C57BL/6 Mice

Human Papillomavirus Type 16 L1 Capsomeres Induce L1-Specific Cytotoxic T Lymphocytes and Tumor Regression in C57BL/6 Mice

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ÖHLSCHLÄGER, Peter, Wolfram OSEN, Kerstin DELL, Stefan FAATH, Robert L. GARCEA, Ingid JOCHMUS, Martin MÜLLER, Michael PAWLITA, Klaus SCHÄFER, Peter SEHR, Caroline STAIB, Gerd SUTTER, Lutz GISSMANN, 2003. Human Papillomavirus Type 16 L1 Capsomeres Induce L1-Specific Cytotoxic T Lymphocytes and Tumor Regression in C57BL/6 Mice. In: Journal of Virology. 77(8), pp. 4635-4645. ISSN 0022-538X. Available under: doi: 10.1128/JVI.77.8.4635-4645.2003

@article{Ohlschlager2003Human-17502, title={Human Papillomavirus Type 16 L1 Capsomeres Induce L1-Specific Cytotoxic T Lymphocytes and Tumor Regression in C57BL/6 Mice}, year={2003}, doi={10.1128/JVI.77.8.4635-4645.2003}, number={8}, volume={77}, issn={0022-538X}, journal={Journal of Virology}, pages={4635--4645}, author={Öhlschläger, Peter and Osen, Wolfram and Dell, Kerstin and Faath, Stefan and Garcea, Robert L. and Jochmus, Ingid and Müller, Martin and Pawlita, Michael and Schäfer, Klaus and Sehr, Peter and Staib, Caroline and Sutter, Gerd and Gissmann, Lutz} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/17502"> <dc:rights>deposit-license</dc:rights> <dc:contributor>Jochmus, Ingid</dc:contributor> <dc:contributor>Osen, Wolfram</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/17502"/> <dcterms:issued>2003</dcterms:issued> <dcterms:abstract xml:lang="eng">We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1165-173 peptide for ex vivo restimulation of splenocytes prior to analysis (51Cr release assay and enzyme-linked immunospot assay [ELISPOT]). This peptide was identified in this study as a Db-restricted naturally processed CTL epitope by HPV16 L1 sequence analysis, major histocompatibility complex class I binding, and 51Cr release assays following immunization of C57BL/6 mice with HPV16 L1 virus-like particles (VLPs). HPV16 L1 capsomeres were obtained by purification of HPV16 L1 lacking 10 N-terminal amino acids after expression in Escherichia coli as a glutathione S-transferase fusion protein (GST-HPV16 L1ΔN10). Sedimentation analysis revealed that the majority of the purified protein consisted of pentameric capsomeres, and assembled particles were not observed in minor contaminating higher-molecular-weight material. Subcutaneous (s.c.) as well as intranasal immunization of C57BL/6 mice with HPV16 L1 capsomeres triggered an L1-specific CTL response in a dose-dependent manner as measured by ELISPOT and 51Cr release assay. Significant reduction of contaminating bacterial endotoxin (lipopolysaccharide) from the capsomere preparation did not diminish the immunogenicity. Antibody responses (serum and vaginal) were less robust under the experimental conditions employed. In addition, s.c. vaccination with HPV16 L1 capsomeres induced regression of established tumors expressing L1 determinants (C3 tumor cells). Our data demonstrate that capsomeres are potent inducers of CTL responses similar to completely assembled T=7 VLPs. This result is of potential relevance for the development of (combined prophylactic and therapeutic) HPV-specific vaccines, since capsomeres can be produced easily and also can be modified to incorporate heterologous sequences such as early HPV proteins.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-18T09:29:44Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Faath, Stefan</dc:contributor> <dc:contributor>Öhlschläger, Peter</dc:contributor> <dc:creator>Osen, Wolfram</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-18T09:29:44Z</dcterms:available> <dcterms:bibliographicCitation>Publ. in: Journal of virology ; 77 (2003), 8. - S. 4635–4645</dcterms:bibliographicCitation> <dc:creator>Staib, Caroline</dc:creator> <dc:creator>Faath, Stefan</dc:creator> <dc:language>eng</dc:language> <dc:creator>Jochmus, Ingid</dc:creator> <dc:contributor>Sehr, Peter</dc:contributor> <dc:contributor>Garcea, Robert L.</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Dell, Kerstin</dc:creator> <dc:creator>Sutter, Gerd</dc:creator> <dc:contributor>Sutter, Gerd</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Müller, Martin</dc:creator> <dc:creator>Schäfer, Klaus</dc:creator> <dc:creator>Öhlschläger, Peter</dc:creator> <dc:contributor>Gissmann, Lutz</dc:contributor> <dc:creator>Gissmann, Lutz</dc:creator> <dc:contributor>Schäfer, Klaus</dc:contributor> <dc:contributor>Müller, Martin</dc:contributor> <dcterms:title>Human Papillomavirus Type 16 L1 Capsomeres Induce L1-Specific Cytotoxic T Lymphocytes and Tumor Regression in C57BL/6 Mice</dcterms:title> <dc:contributor>Pawlita, Michael</dc:contributor> <dc:creator>Garcea, Robert L.</dc:creator> <dc:contributor>Dell, Kerstin</dc:contributor> <dc:creator>Sehr, Peter</dc:creator> <dc:creator>Pawlita, Michael</dc:creator> <dc:contributor>Staib, Caroline</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>

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