KOPS - Das Institutionelle Repositorium der Universität Konstanz

GFAP-independent inflammatory competence and trophic functions of astrocytes generated from murine embryonic stem cells

GFAP-independent inflammatory competence and trophic functions of astrocytes generated from murine embryonic stem cells

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:4698f45e1a018a1531e8a3b51d18e098

KÜGLER, Philipp, Bettina BAUMANN, Bastian ZIMMER, Sascha KELLER, Andreas MARX, Suzanne KADEREIT, Marcel LEIST, 2012. GFAP-independent inflammatory competence and trophic functions of astrocytes generated from murine embryonic stem cells. In: Glia. 60(2), pp. 218-228. ISSN 0894-1491. eISSN 1098-1136. Available under: doi: 10.1002/glia.21257

@article{Kugler2012-02GFAPi-17413, title={GFAP-independent inflammatory competence and trophic functions of astrocytes generated from murine embryonic stem cells}, year={2012}, doi={10.1002/glia.21257}, number={2}, volume={60}, issn={0894-1491}, journal={Glia}, pages={218--228}, author={Kügler, Philipp and Baumann, Bettina and Zimmer, Bastian and Keller, Sascha and Marx, Andreas and Kadereit, Suzanne and Leist, Marcel} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/17413"> <dc:creator>Zimmer, Bastian</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/17413"/> <dc:creator>Keller, Sascha</dc:creator> <dc:contributor>Kadereit, Suzanne</dc:contributor> <dc:creator>Marx, Andreas</dc:creator> <dc:contributor>Zimmer, Bastian</dc:contributor> <dc:contributor>Keller, Sascha</dc:contributor> <dc:contributor>Kügler, Philipp</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/17413/2/Leist%20GFAP.pdf"/> <dcterms:title>GFAP-independent inflammatory competence and trophic functions of astrocytes generated from murine embryonic stem cells</dcterms:title> <dc:creator>Kügler, Philipp</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-10-31T23:25:05Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Baumann, Bettina</dc:contributor> <dcterms:abstract xml:lang="eng">The directed generation of pure astrocyte cultures from pluripotent stem cells has proven difficult. Generation of defined pluripotent-stem-cell derived astrocytes would allow new approaches to the investigation of plasticity and heterogeneity of astrocytes. We here describe a two-step differentiation scheme resulting in the generation of murine embryonic stem cell (mESC) derived astrocytes (MEDA), as characterized by the upregulation of 19 astrocyte-associated mRNAs, and positive staining of most cells for GFAP (glial fibrillary acidic protein), aquaporin-4 or glutamine synthetase. The MEDA cultures could be cryopreserved, and they neither contained neuronal, nor microglial cells. They also did not react to the microglial stimulus lipopolysaccharide, while inflammatory activation by a complete cytokine mix (CCM) or its individual components (TNF-α, IL1-β, IFN-γ) was readily observed. MEDA, stimulated by CCM, became susceptible to CD95 ligand-induced apoptosis and produced NO and IL-6. This was preceded by NF-kB activation, and up-regulation of relevant mRNAs. Also GFAP-negative astrocytes were fully inflammation-competent. Neurotrophic support by MEDA was found to be independent of GFAP expression. In summary, we described here the generation and functional characterization of microglia-free murine astrocytes, displaying phenotypic heterogeneity as is commonly observed in brain astrocytes</dcterms:abstract> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:rights>deposit-license</dc:rights> <dc:creator>Baumann, Bettina</dc:creator> <dc:contributor>Marx, Andreas</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-13T08:14:57Z</dc:date> <dc:creator>Kadereit, Suzanne</dc:creator> <dcterms:issued>2012-02</dcterms:issued> <dc:creator>Leist, Marcel</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:bibliographicCitation>First publ. in: Glia ; 60 (2012), 2. - S. 218-228</dcterms:bibliographicCitation> <dc:language>eng</dc:language> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/17413/2/Leist%20GFAP.pdf"/> <dc:contributor>Leist, Marcel</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Leist GFAP.pdf 483

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto