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Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons

Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons

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Prüfsumme: MD5:3238a07380e8065e9b99b9d74668c6b7

BICKER, Gerd, Sabine KREISSL, 1994. Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons. In: Journal of Neurophysiology. 71(2), pp. 808-810

@article{Bicker1994Calci-17300, title={Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons}, year={1994}, number={2}, volume={71}, journal={Journal of Neurophysiology}, pages={808--810}, author={Bicker, Gerd and Kreißl, Sabine} }

Kreißl, Sabine First publ. in: Journal of Neurophysiology ; 71 (1994), 2. - pp. 808-810 eng Bicker, Gerd deposit-license Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons 1. We used fluorescence imaging with the visible wavelength indicator fluo-3 to investigate the calcium responses to cholinergic ligands of honeybee Kenyon cells in primary culture.<br />2. Application of acetylcholine ( ACh) or nicotine, but not pilocarpine, promoted a calcium influx into the cell body and neurites. The increase in intracellular calcium after ACh stimulation was blocked by tu-bungarotoxin. These results support previous histochemical studies that suggested the expression of nicotinic cholinergic receptors on Kenyon cells.<br />3. After depolarization with high K+ solution fluorescence increased<br />in the somata and neurites, which indicates the presence of<br />voltage-gated Ca *+ channels in Kenyon cell membranes. 2012-01-31T11:59:36Z Bicker, Gerd Kreißl, Sabine 1994 2012-01-31T11:59:36Z

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