Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons

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1994
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Bicker, Gerd
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Published
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Journal of Neurophysiology. 1994, 71(2), pp. 808-810
Zusammenfassung
  1. We used fluorescence imaging with the visible wavelength indicator fluo-3 to investigate the calcium responses to cholinergic ligands of honeybee Kenyon cells in primary culture.
    2. Application of acetylcholine ( ACh) or nicotine, but not pilocarpine, promoted a calcium influx into the cell body and neurites. The increase in intracellular calcium after ACh stimulation was blocked by tu-bungarotoxin. These results support previous histochemical studies that suggested the expression of nicotinic cholinergic receptors on Kenyon cells.
    3. After depolarization with high K+ solution fluorescence increased
    in the somata and neurites, which indicates the presence of
    voltage-gated Ca *+ channels in Kenyon cell membranes.
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570 Biowissenschaften, Biologie
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ISO 690BICKER, Gerd, Sabine KREISSL, 1994. Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons. In: Journal of Neurophysiology. 1994, 71(2), pp. 808-810
BibTex
@article{Bicker1994Calci-17300,
  year={1994},
  title={Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons},
  number={2},
  volume={71},
  journal={Journal of Neurophysiology},
  pages={808--810},
  author={Bicker, Gerd and Kreißl, Sabine}
}
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