Screen for kinases affecting amyloidogenic cleavage by BACE1

Cite This

Files in this item

Checksum: MD5:2d071b84f385782935aeffefa8ec4c64

PENZKOFER, Stephan, 2011. Screen for kinases affecting amyloidogenic cleavage by BACE1 [Dissertation]. Konstanz: University of Konstanz

@phdthesis{Penzkofer2011Scree-17287, title={Screen for kinases affecting amyloidogenic cleavage by BACE1}, year={2011}, author={Penzkofer, Stephan}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:title>Screen for kinases affecting amyloidogenic cleavage by BACE1</dcterms:title> <dspace:hasBitstream rdf:resource=""/> <dc:date rdf:datatype="">2011-12-05T11:34:37Z</dc:date> <bibo:uri rdf:resource=""/> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:abstract xml:lang="eng">The Amyloid β peptide (Aβ) is suspected to be a causal agent for Alzheimer’s disease (AD). Therefore a screen for kinases downregulating the initial step of its production, the cleavage of the Amyloid Precursor Protein (APP) by Beta-site of APP Cleaving Enzyme 1 (BACE1), was conducted in this study. Briefly, HEK293 cells were colipofected with one of in total 1357 siRNAs against 60% of the human kinome and either an APP construct with only the β-cleavage site left or normally cleavable APP as control. Remaining β-cleavage was for logistic reasons firstly measured with an activity-test for secreted alkaline phosphatase (SEAP) fused to both types of APP and subjected to Aβ-ELISA when interesting. Before the screen, the APP-constructs were characterized in the cell types HEK293 and CGCs with regards to cleavage, especially by BACE1. The screen resulted in 38 hits of which one, Testis Specific Serine Kinase 3, was confirmed once more. In a second, bioinformatic project, an initially suspected APLP-like pseudogenic-like sequence in C3orf52 was refuted. Further, analysis of C3orf52 gene expression data hints on a role in myeloid leukemia. Lastly, the phylogenetic relationship of the APP family paralogs was examined, also in comparison to neighboring gene families, and found in the topology (APLP1)(APLP2/APP).</dcterms:abstract> <dcterms:isPartOf rdf:resource=""/> <dcterms:hasPart rdf:resource=""/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Penzkofer, Stephan</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="">2011-12-05T11:34:37Z</dcterms:available> <dcterms:rights rdf:resource=""/> <dcterms:issued>2011</dcterms:issued> <dc:creator>Penzkofer, Stephan</dc:creator> <dc:language>eng</dc:language> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Diss_Penzkofer.pdf 2364

This item appears in the following Collection(s)

Search KOPS


My Account