Differential potencies of effector genes in adult Drosophila

Cite This

Files in this item

Checksum: MD5:d3f02264a519625c686a0bead47bc9fb

THUM, Andreas, Stephan KNAPEK, Jens RISTER, Eva DIERICHS-SCHMITT, Martin HEISENBERG, Hiromu TANIMOTO, 2006. Differential potencies of effector genes in adult Drosophila. In: The Journal of Comparative Neurology. 498(2), pp. 194-203. ISSN 0021-9967. Available under: doi: 10.1002/cne.21022

@article{Thum2006-09-10Diffe-17146, title={Differential potencies of effector genes in adult Drosophila}, year={2006}, doi={10.1002/cne.21022}, number={2}, volume={498}, issn={0021-9967}, journal={The Journal of Comparative Neurology}, pages={194--203}, author={Thum, Andreas and Knapek, Stephan and Rister, Jens and Dierichs-Schmitt, Eva and Heisenberg, Martin and Tanimoto, Hiromu} }

Tanimoto, Hiromu Heisenberg, Martin 2011-11-29T12:43:13Z Dierichs-Schmitt, Eva eng Knapek, Stephan Tanimoto, Hiromu Differential potencies of effector genes in adult Drosophila Knapek, Stephan First publ. in: The Journal of Comparative Neurology ; 498 (2006), 2. - S.194-203 Dierichs-Schmitt, Eva Rister, Jens 2006-09-10 2011-11-29T12:43:13Z Heisenberg, Martin terms-of-use Rister, Jens Thum, Andreas Thum, Andreas The GAL4/UAS gene expression system in Drosophila has been crucial in revealing the behavioral significance of neural circuits. Transgene products that block neurotransmitter release and induce cell death have been proved to inhibit neural function powerfully. Here we compare the action of the five effector genes shibire<sup>ts1</sup>, Tetanus toxin light chain (TNT), reaper, Diphtheria toxin A-chain (DTA), and inwardly rectifying potassium channel (Kir2.1) and show differences in their efficiency depending on the target cells and the timing of induction. Specifically, effectors blocking neuronal transmission or excitability led to adultinduced paralysis more efficiently than those causing cell ablation. We contrasted these differential potencies in adult to their actions during development. Furthermore, we induced TNT expression in the adult mushroom bodies. In contrast to the successful impairment in short-term olfactory memory by shibire<sup>ts1</sup>, adult TNT expression in the same set of cells did not lead to any obvious impairment. Altogether, the efficiency of effector genes depends on properties of the targeted neurons. Thus, we conclude that the selection of the appropriate effector gene is critical for evaluating the function of neural circuits.

Downloads since Oct 1, 2014 (Information about access statistics)

Thum etal.pdf 467

This item appears in the following Collection(s)

Search KOPS


My Account