Force-induced structural transitions in cross-linked DNA films


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ANDRÉ, Alexander, Fabien FONTAINE-VIVE, Heiko MÖLLER, Theo FISCHER, Georg MARET, Thomas GISLER, V. Trevor FORSYTH, 2008. Force-induced structural transitions in cross-linked DNA films. In: European Biophysics Journal. 37(6), pp. 749-757. ISSN 0175-7571

@article{Andre2008-07Force-16876, title={Force-induced structural transitions in cross-linked DNA films}, year={2008}, doi={10.1007/s00249-008-0257-4}, number={6}, volume={37}, issn={0175-7571}, journal={European Biophysics Journal}, pages={749--757}, author={André, Alexander and Fontaine-Vive, Fabien and Möller, Heiko and Fischer, Theo and Maret, Georg and Gisler, Thomas and Forsyth, V. Trevor} }

<rdf:RDF xmlns:rdf="" xmlns:bibo="" xmlns:dc="" xmlns:dcterms="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Forsyth, V. Trevor</dc:contributor> <dcterms:bibliographicCitation>First publ. in: European Biophysics Journal ; 37 (2008), 6. - S. 749-757</dcterms:bibliographicCitation> <dc:creator>Fontaine-Vive, Fabien</dc:creator> <dc:contributor>André, Alexander</dc:contributor> <dcterms:title>Force-induced structural transitions in cross-linked DNA films</dcterms:title> <dcterms:available rdf:datatype="">2012-04-02T12:58:42Z</dcterms:available> <dc:creator>André, Alexander</dc:creator> <dcterms:issued>2008-07</dcterms:issued> <dc:creator>Maret, Georg</dc:creator> <dc:creator>Gisler, Thomas</dc:creator> <dc:contributor>Fontaine-Vive, Fabien</dc:contributor> <dc:contributor>Maret, Georg</dc:contributor> <dc:creator>Möller, Heiko</dc:creator> <dc:contributor>Möller, Heiko</dc:contributor> <dcterms:abstract xml:lang="eng">We report on the preparation and characterization of wet-spun films of sodium DNA in which intermolecular cross-links were introduced following formaldehyde treatment. Raman scattering shows that the DNA in moderately cross-linked films is mainly in the B conformation. Stretching experiments show a transition from plastic to elastomeric behavior with increasing exposure to the cross-linking agent. Elastomeric DNA films are strongly disordered. X-ray diffraction shows that stretching of moderately cross-linked films under controlled high humidity conditions results in increased molecular orientation as well as the appearance of meridional reflections at 7.4–7.8 and 8.2 Å. These reflections are not observed for any of the classical conformations associated with mixed sequence DNA, and may arise from extended base-pair stacking in a stretched DNA structure.</dcterms:abstract> <dc:creator>Forsyth, V. Trevor</dc:creator> <dc:language>eng</dc:language> <dc:rights>deposit-license</dc:rights> <dc:contributor>Fischer, Theo</dc:contributor> <dcterms:rights rdf:resource=""/> <bibo:uri rdf:resource=""/> <dc:date rdf:datatype="">2012-04-02T12:58:42Z</dc:date> <dc:creator>Fischer, Theo</dc:creator> <dc:contributor>Gisler, Thomas</dc:contributor> </rdf:Description> </rdf:RDF>

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