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Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry

Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry

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WU, Bei, Iuliana SUSNEA, Yingxu CHEN, Michael PRZYBYLSKI, J. Sabine BECKER, 2011. Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry. In: International Journal of Mass Spectrometry. 307(1-3), pp. 85-91. ISSN 1387-3806

@article{Wu2011Study-16773, title={Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry}, year={2011}, doi={10.1016/j.ijms.2011.01.018}, number={1-3}, volume={307}, issn={1387-3806}, journal={International Journal of Mass Spectrometry}, pages={85--91}, author={Wu, Bei and Susnea, Iuliana and Chen, Yingxu and Przybylski, Michael and Becker, J. Sabine} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/16773"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16773"/> <dcterms:bibliographicCitation>First publ. in: International Journal of Mass Spectrometry ; 307 (2011), 1-3. - pp. 85-91</dcterms:bibliographicCitation> <dc:creator>Becker, J. Sabine</dc:creator> <dcterms:title>Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry</dcterms:title> <dc:creator>Susnea, Iuliana</dc:creator> <dcterms:issued>2011</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-12T08:48:50Z</dcterms:available> <dc:creator>Chen, Yingxu</dc:creator> <dc:contributor>Becker, J. Sabine</dc:contributor> <dc:contributor>Wu, Bei</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Przybylski, Michael</dc:creator> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">In the present study, the metal-containing proteins in the roots of Cu-tolerant plant Elsholtzia splendens were investigated. The proteins from the plant roots were extracted and separated by two-dimensional gel electrophoresis (2D GE). Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to screen metal-containing proteins on the dried 2D gels. Images of Cu, Zn, Na, Mg, K, Ca, Mn, Fe, P and S, and also of Ag, were obtained by scanning the gel (line by line) using the LA-ICP-MS techniques developed in our laboratory. Cu-, Zn- and Mg-containing proteins were found in the gel sections of interest via LA-ICP-MS imaging. These protein spots were then in-gel digested with trypsin and analyzed by liquid chromatography tandem mass spectrometry (LC–MS/MS) for identification. The mechanisms involved in Cu tolerance and accumulation were also discussed.</dcterms:abstract> <dc:creator>Wu, Bei</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-12T08:48:50Z</dc:date> <dc:contributor>Susnea, Iuliana</dc:contributor> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:contributor>Chen, Yingxu</dc:contributor> </rdf:Description> </rdf:RDF>

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