KOPS - Das Institutionelle Repositorium der Universität Konstanz

Coordinated waves of gene expression during neuronal differentiation of embryonic stem cells as basis for novel approaches to developmental neurotoxicity testing

Coordinated waves of gene expression during neuronal differentiation of embryonic stem cells as basis for novel approaches to developmental neurotoxicity testing

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:c0bfd4d61c8ee39b410e2f4fc0abb049

ZIMMER, Bastian, Philipp KÜGLER, Birte BAUDIS, Andreas GENEWSKY, Vivek TANAVDE, W. KOH, B. TAN, Tanja WALDMANN, Suzanne KADEREIT, Marcel LEIST, 2011. Coordinated waves of gene expression during neuronal differentiation of embryonic stem cells as basis for novel approaches to developmental neurotoxicity testing. In: Cell Death and Differentiation. 18(3), pp. 383-395. ISSN 1350-9047. eISSN 1476-5403

@article{Zimmer2011-03Coord-16733, title={Coordinated waves of gene expression during neuronal differentiation of embryonic stem cells as basis for novel approaches to developmental neurotoxicity testing}, year={2011}, doi={10.1038/cdd.2010.109}, number={3}, volume={18}, issn={1350-9047}, journal={Cell Death and Differentiation}, pages={383--395}, author={Zimmer, Bastian and Kügler, Philipp and Baudis, Birte and Genewsky, Andreas and Tanavde, Vivek and Koh, W. and Tan, B. and Waldmann, Tanja and Kadereit, Suzanne and Leist, Marcel} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/16733"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-12-06T07:14:02Z</dc:date> <dc:contributor>Tan, B.</dc:contributor> <dc:creator>Koh, W.</dc:creator> <dc:creator>Baudis, Birte</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Kadereit, Suzanne</dc:contributor> <dc:contributor>Genewsky, Andreas</dc:contributor> <dc:creator>Zimmer, Bastian</dc:creator> <dc:creator>Tanavde, Vivek</dc:creator> <dc:contributor>Waldmann, Tanja</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-12-06T07:14:02Z</dcterms:available> <dcterms:bibliographicCitation>First publ. in: Cell Death and Differentiation ; 18 (2011), 3. - pp. 383–395</dcterms:bibliographicCitation> <dc:contributor>Leist, Marcel</dc:contributor> <dc:creator>Tan, B.</dc:creator> <dc:creator>Leist, Marcel</dc:creator> <dcterms:title>Coordinated waves of gene expression during neuronal differentiation of embryonic stem cells as basis for novel approaches to developmental neurotoxicity testing</dcterms:title> <dcterms:issued>2011-03</dcterms:issued> <dc:contributor>Koh, W.</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:abstract xml:lang="eng">As neuronal differentiation of embryonic stem cells (ESCs) recapitulates embryonic neurogenesis, disturbances of this process may model developmental neurotoxicity (DNT). To identify the relevant steps of in vitro neurodevelopment, we implemented a differentiation protocol yielding neurons with desired electrophysiological properties. Results from focussed transcriptional profiling suggested that detection of non-cytotoxic developmental disturbances triggered by toxicants such as retinoic acid (RA) or cyclopamine was possible. Therefore, a broad transcriptional profile of the 20-day differentiation process was obtained. Cluster analysis of expression kinetics, and bioinformatic identification of overrepresented gene ontologies revealed waves of regulation relevant for DNT testing. We further explored the concept of superimposed waves as descriptor of ordered, but overlapping biological processes. The initial wave of transcripts indicated reorganization of chromatin and epigenetic changes. Then, a transient upregulation of genes involved in the formation and patterning of neuronal precursors followed. Simultaneously, a long wave of ongoing neuronal differentiation started. This was again superseded towards the end of the process by shorter waves of neuronal maturation that yielded information on specification, extracellular matrix formation, disease-associated genes and the generation of glia. Short exposure to lead during the final differentiation phase, disturbed neuronal maturation. Thus, the wave kinetics and the patterns of neuronal specification define the time windows and end points for examination of DNT.</dcterms:abstract> <dc:contributor>Tanavde, Vivek</dc:contributor> <dc:contributor>Zimmer, Bastian</dc:contributor> <dc:creator>Kadereit, Suzanne</dc:creator> <dc:creator>Genewsky, Andreas</dc:creator> <dc:contributor>Kügler, Philipp</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Baudis, Birte</dc:contributor> <dc:creator>Waldmann, Tanja</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16733"/> <dc:creator>Kügler, Philipp</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Leist_Coordinated waves.pdf 131

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto