Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-167259 |
| Author: | Sauter, Katharina B.; Marx, Andreas |
| Year of publication: | 2006 |
| Published in: | Angewandte Chemie International Edition ; 45 (2006), 45. - pp. 7633-7635. - ISSN 1433-7851 |
| Pubmed ID: | 17054304 |
| DOI (citable link): | https://dx.doi.org/10.1002/anie.200602772 |
| Summary: |
Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcribe RNA into DNA. We show that enzymes with significantly increased reverse transcription ability can be generated by directed evolution.
|
| Subject (DDC): | 540 Chemistry |
| Keywords: | directed evolution, DNA polymerase, DNA recognition, polymerase chain reaction, reverse transcriptase |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
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SAUTER, Katharina B., Andreas MARX, 2006. Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold. In: Angewandte Chemie International Edition. 45(45), pp. 7633-7635. ISSN 1433-7851. Available under: doi: 10.1002/anie.200602772
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