@article{Askew1999Chara-16550,
  year={1999},
  title={Characterization of Apt- cell lines exhibiting crossresistance to glucocorticoid- and Fas-mediated apoptosis},
  number={8},
  volume={6},
  journal={Cell Death and Differentiation},
  pages={796--804},
  author={Askew, David and Kuscuoglu, Unsal and Brunner, Thomas and Green, Douglas R. and Miesfeld, Roger L.}
}

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    <dc:contributor>Brunner, Thomas</dc:contributor>
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    <dcterms:abstract xml:lang="eng">Apoptosis induction by staurosporine, ceramide, and Fas stimulation was investigated in the mouse thymoma cell line W7.2 and a panel of dexamethasone (dex)-resistant W7.2 mutant cell lines, Apt3.8, Apt4.8 and Apt5.8, and a Bcl-2 transfectedW7.2 cell line (Wbcl2).WhileW7.2 cellswere found to be sensitive to these apoptosis inducers, the Apt- mutants andWbcl2 cells were shownto be resistant tosome or all of the treatments.Specifically, all threeApt-mutantsandWbcl2cells were found to be resistant to ceramide and Fas-mediated apoptosis, whereas, Apt4.8 and Apt5.8 were sensitive to staurosporine-induced apoptosis under conditions in which Apt3.8 and Wbcl2 cells were resistant. Measurements of caspase activity and cytochrome c release in cytosolic extracts of dex and staurosporine-treated cells indicated that the recessive Apt- mutations effect steps upstream of mitochondrial dysfunction. Steady-state RNA levels of apoptosis-associated gene transcripts showed that the observed differential resistance of the Apt- cell lines could not be explained by altered expression of numerous Bcl-2 or Fas related genes. Transient transfection of human Fas gene coding sequences into the Apt- mutants and Wbcl2 cells did not induce apoptosis, even though these same cell lines were sensitive to ectopic expression of the FADD and caspase 8 genes. Taken together, these data provide genetic evidence for the existence of shared components in the dex- and Fasmediated apoptotic pathways in W7.2 cells.</dcterms:abstract>
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    <dc:creator>Askew, David</dc:creator>
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    <dc:contributor>Kuscuoglu, Unsal</dc:contributor>
    <dc:creator>Miesfeld, Roger L.</dc:creator>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-02T11:45:21Z</dc:date>
    <dc:contributor>Green, Douglas R.</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:bibliographicCitation>First publ. in: Cell Death and Differentiation ; 6 (1999), 8. - pp. 796-804</dcterms:bibliographicCitation>
    <dc:contributor>Askew, David</dc:contributor>
    <dcterms:title>Characterization of Apt- cell lines exhibiting crossresistance to glucocorticoid- and Fas-mediated apoptosis</dcterms:title>
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    <dc:creator>Kuscuoglu, Unsal</dc:creator>
    <dc:contributor>Miesfeld, Roger L.</dc:contributor>
    <dc:creator>Green, Douglas R.</dc:creator>
    <dc:creator>Brunner, Thomas</dc:creator>
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