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Photochemical release of ATP from "Caged ATP" studied by time-resolved infrared spectroscopy

Photochemical release of ATP from "Caged ATP" studied by time-resolved infrared spectroscopy

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Prüfsumme: MD5:39949601a49714779d1f59180f9eb45c

BARTH, Andreas, Karin HAUSER, Werner MAENTELE, John E. T. CORRIE, David R. TRENTHAM, 1995. Photochemical release of ATP from "Caged ATP" studied by time-resolved infrared spectroscopy. In: Journal of the American Chemical Society. 117(41), pp. 10311-10316. ISSN 0002-7863

@article{Barth1995Photo-16460, title={Photochemical release of ATP from "Caged ATP" studied by time-resolved infrared spectroscopy}, year={1995}, doi={10.1021/ja00146a015}, number={41}, volume={117}, issn={0002-7863}, journal={Journal of the American Chemical Society}, pages={10311--10316}, author={Barth, Andreas and Hauser, Karin and Maentele, Werner and Corrie, John E. T. and Trentham, David R.} }

Photochemical release of ATP from "Caged ATP" studied by time-resolved infrared spectroscopy Maentele, Werner Maentele, Werner Corrie, John E. T. 2012-01-05T12:19:14Z 2012-01-05T12:19:14Z Hauser, Karin Barth, Andreas 1995 First publ. in: Journal of the American Chemical Society ; 117 (1995), 41. - pp 10311–10316 Rapid scan Fourier transform infrared (FTIR) spectroscopy and time-resolved single wavelength infrared (IR) spectroscopy have been used to follow the photochemical release of adenosine 5’-triphosphate (ATP) from P3-( 1 -(2-nitrophenyl)ethyl) adenosine 5’-triphosphate (caged ATP). Vibrational difference spectra for the formation first of the aci-nitro anion intermediate and subsequently of ATP and the byproduct(s) were obtained by rapid scan FTIR spectroscopy in the millisecond-to-second time domain. Vibrational modes of the phosphate groups of ATP and caged ATP in the range 1250-900 cm-I could be assigned on the basis of triple and single l8O labeling in caged ATP at the terminal phosphate group and at the bridging oxygen between the terminal phosphate and the 1-(2-nitrophenyl)ethyl group, respectively. The rapid formation and subsequent decay of the aci-nitro anion intermediate were monitored by single-wavelength time-resolved IR spectroscopy at 125 1 cm- ’ (predominantly a PO2- mode of caged ATP and the aci-nitro intermediate). The appearance of the free y-phosphate group of ATP was monitored at 1119 cm-I (P03’- mode of ATP). Decay of the aci-nitro anion intermediate and formation of ATP were well fitted by single exponentials to give a mean rate constant of 218 & 33 s-’ at pH 7 and 22 OC. Hauser, Karin deposit-license Corrie, John E. T. Trentham, David R. eng Trentham, David R. Barth, Andreas

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