Diamondoid-modified DNA


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WANG, Yan, Boryslav A. TKACHENKO, Peter R. SCHREINER, Andreas MARX, 2011. Diamondoid-modified DNA. In: Organic & Biomolecular Chemistry. 9(21), pp. 7482-7490. ISSN 1477-0520. eISSN 1477-0539

@article{Wang2011-11-07Diamo-16359, title={Diamondoid-modified DNA}, year={2011}, doi={10.1039/c1ob05929g}, number={21}, volume={9}, issn={1477-0520}, journal={Organic & Biomolecular Chemistry}, pages={7482--7490}, author={Wang, Yan and Tkachenko, Boryslav A. and Schreiner, Peter R. and Marx, Andreas} }

Schreiner, Peter R. Wang, Yan deposit-license 2012-08-31T22:25:04Z Marx, Andreas eng Tkachenko, Boryslav A. Wang, Yan 2011-10-26T11:00:15Z First publ. in: Organic & Biomolecular Chemistry ; 9 (2011), 21. - S. 7482-7490 Diamondoid-modified DNA We prepared novel C5-modified triphosphates and phosphoramidites with a diamondoid functionally linked to the nucleobase. Using primer extension experiments with different length templates we investigated whether the modified triphosphates were enzymatically incorporated into DNA and whether they were further extended. We found that all three modified nucleotides can be incorporated into DNA using a single-nucleotide incorporation experiment, but only partially using two templates that demand for multiple incorporation of the modified nucleotides. The modified phosphoramidites were introduced into oligonucleotides utilizing DNA synthesizer technology. The occurring oligonucleotide structures were examined by circular dichroism (CD) and melting temperature (Tm)measurements and were found to adapt similar helix conformations as their unmodified counterparts. Marx, Andreas Schreiner, Peter R. Tkachenko, Boryslav A. 2011-11-07

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