Screening of molecular interactions using reporter hammerhead ribozymes

Cite This

Files in this item

Checksum: MD5:c269af9f3533d6abbfc45330484dad00

HARTIG, Jörg, Michael FAMULOK, 2008. Screening of molecular interactions using reporter hammerhead ribozymes. In: MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols. Totowa, NJ:Humana Press, pp. 251-263. ISBN 978-1-58829-700-6. Available under: doi: 10.1007/978-1-60327-040-3_18

@incollection{Hartig2008Scree-15681, title={Screening of molecular interactions using reporter hammerhead ribozymes}, year={2008}, doi={10.1007/978-1-60327-040-3_18}, number={429}, isbn={978-1-58829-700-6}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols}, pages={251--263}, editor={Marx, Andreas and Seitz, Oliver}, author={Hartig, Jörg and Famulok, Michael} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:language>eng</dc:language> <dcterms:title>Screening of molecular interactions using reporter hammerhead ribozymes</dcterms:title> <dspace:hasBitstream rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Famulok, Michael</dc:contributor> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:hasPart rdf:resource=""/> <dcterms:rights rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource=""/> <dc:creator>Famulok, Michael</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dc:date rdf:datatype="">2011-10-10T12:05:21Z</dc:date> <dcterms:abstract xml:lang="eng">The characterization of molecular interactions is a central task in modern life sciences. Applications such as drug screening in pharmaceutics or the elucidation of biomolecular interactions in molecular biology rely on efficient methods to search for interacting partners. Here, we describe a novel technique that utilizes hammerhead ribozymes to signal molecular interactions. The ribozyme is modified by a domain that specifically binds to a target molecule such as a protein. Upon binding of the target, the catalytic activity of the ribozyme is changed, allowing for detection of the presence as well as the occurrence of interactions of the targeted ligand. The assay can be performed in high-throughput format by employing double-labeled ribozyme substrates, hence being well suited for drug-screening applications. The detection proceeds rapidly and in real-time. Moreover, the technique neither requires labeling of the target molecule nor the potential interaction partners or analytes since an indirect readout is facilitated by switching the catalytic activity of a reporter ribozyme. The assay can be utilized to sense a broad variety of biomolecular interactions, and is very sensitive due to signal amplification by the ribozyme reaction</dcterms:abstract> <dcterms:issued>2008</dcterms:issued> <dcterms:bibliographicCitation>Molecular Beacons : signalling Nucleic Acid Probes Methods, and Protocols / Andreas Marx .... - Totowa, NJ : Humana Press, 2008. - pp. 251-263. - (Methods in molecular biology ; 429). - ISBN 978-1-58829-700-6</dcterms:bibliographicCitation> <dc:creator>Hartig, Jörg</dc:creator> <bibo:uri rdf:resource=""/> <dc:contributor>Hartig, Jörg</dc:contributor> <dcterms:available rdf:datatype="">2011-10-10T12:05:21Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Hartig_156817.pdf 356

This item appears in the following Collection(s)

Search KOPS


My Account