Protein-dependent ribozymes report molecular interactions in real time

Cite This

Files in this item

Checksum: MD5:ba2c75c54599e4423d523656ffbd8961

HARTIG, Jörg, S. Hani NAJAFI-SHOUSHTARI, Imke GRÜNE, Amy YAN, Andrew D. ELLINGTON, Michael FAMULOK, 2002. Protein-dependent ribozymes report molecular interactions in real time. In: Nature Biotechnology. 20(7), pp. 717-722. ISSN 1087-0156. Available under: doi: 10.1038/nbt0702-717

@article{Hartig2002-07Prote-15661, title={Protein-dependent ribozymes report molecular interactions in real time}, year={2002}, doi={10.1038/nbt0702-717}, number={7}, volume={20}, issn={1087-0156}, journal={Nature Biotechnology}, pages={717--722}, author={Hartig, Jörg and Najafi-Shoushtari, S. Hani and Grüne, Imke and Yan, Amy and Ellington, Andrew D. and Famulok, Michael} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:isPartOf rdf:resource=""/> <dcterms:title>Protein-dependent ribozymes report molecular interactions in real time</dcterms:title> <dc:creator>Ellington, Andrew D.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:hasPart rdf:resource=""/> <dspace:isPartOfCollection rdf:resource=""/> <dc:creator>Famulok, Michael</dc:creator> <dc:creator>Yan, Amy</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Ellington, Andrew D.</dc:contributor> <dc:date rdf:datatype="">2011-10-10T10:47:11Z</dc:date> <dcterms:abstract xml:lang="eng">Most approaches to monitoring interactions between biological macromolecules require large amounts of material, rely upon the covalent modification of an interaction partner, or are not amenable to real-time detection. We have developed a generalizable assay system based on interactions between proteins and reporter ribozymes. The assay can be configured in a modular fashion to monitor the presence and concentration of a protein or of molecules that modulate protein function. We report two applications of the assay: screening for a small molecule that disrupts protein binding to its nucleic acid target and screening for protein-protein interactions. We screened a structurally diverse library of antibiotics for small molecules that modulate the activity of HIV-1 Rev-responsive ribozymes by binding to Rev. We identified an inhibitor that subsequently inhibited HIV-1 replication in cells. A simple format switch allowed reliable monitoring of domain-specific interactions between the blood-clotting factor thrombin and its protein partners. The rapid identification of interactions between proteins or of compounds that disrupt such interactions should have substantial utility for the drug-discovery process.</dcterms:abstract> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>2002-07</dcterms:issued> <dc:contributor>Hartig, Jörg</dc:contributor> <dcterms:bibliographicCitation>Nature Biotechnology ; 20 (2002), 7. - S. 717-722</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="">2011-10-10T10:47:11Z</dcterms:available> <dc:contributor>Najafi-Shoushtari, S. Hani</dc:contributor> <dc:contributor>Yan, Amy</dc:contributor> <dcterms:rights rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource=""/> <dc:creator>Hartig, Jörg</dc:creator> <dc:contributor>Grüne, Imke</dc:contributor> <dc:creator>Najafi-Shoushtari, S. Hani</dc:creator> <dc:contributor>Famulok, Michael</dc:contributor> <dspace:hasBitstream rdf:resource=""/> <dc:creator>Grüne, Imke</dc:creator> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Hartig_156618.pdf 201

This item appears in the following Collection(s)

Search KOPS


My Account