KOPS - Das Institutionelle Repositorium der Universität Konstanz

Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase

Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:67e5991b7f351ca215594f6215f46de9

HARTIG, Jörg, Eric T. KOOL, 2005. Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase. In: Nucleic Acids Research. 33(15), pp. 4922-4927. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gki803

@article{Hartig2005Effic-15629, title={Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase}, year={2005}, doi={10.1093/nar/gki803}, number={15}, volume={33}, issn={0305-1048}, journal={Nucleic Acids Research}, pages={4922--4927}, author={Hartig, Jörg and Kool, Eric T.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/15629"> <dc:rights>deposit-license</dc:rights> <dc:creator>Kool, Eric T.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/15629/2/Hartig_156291.pdf"/> <dcterms:bibliographicCitation>Nucleic acids research ; 33 (2005), 15.- pp. 4922-4927</dcterms:bibliographicCitation> <dcterms:issued>2005</dcterms:issued> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/15629/2/Hartig_156291.pdf"/> <dc:contributor>Kool, Eric T.</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:abstract xml:lang="eng">Repeating DNA sequences, such as telomeres, centromeres, and micro- and mini-satellites, comprise 50% of the genome and play important roles in regulatory and pathogenic mechanisms. In order to study structures and functions of such repeating sequences, it is important to have simple and efficient methods for making them in vitro. Here, we describe the efficient and convenient expansion of repetitive telomeric and minisatellite DNA sequences starting from small synthetic templates to final product lengths of several hundreds to thousands of nucleotides by the thermostable DNA polymerase from Thermococcus litoralis (Vent DNA polymerase). This enzyme was so far unknown to catalyze repeat expansion. Either single-stranded or double-stranded DNAs could be produced, depending on nucleotides present. Compared to earlier results obtained with other enzymes, the expansion reaction is highly efficient both in its yield and product length, and proceeds without thermal cycling. Moreover, the products are characterized by a narrow length distribution.</dcterms:abstract> <dcterms:title>Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase</dcterms:title> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-07T13:34:05Z</dc:date> <dc:contributor>Hartig, Jörg</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-07T13:34:05Z</dcterms:available> <dc:creator>Hartig, Jörg</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/15629"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Hartig_156291.pdf 42

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto